银杏幼苗对水分胁迫的生理响应  

Physiological Response to the Different Levels of Water Stress in Seedlings of Ginkgo biloba

作　　者：陈颖[1] 谢寅峰[1] 沈惠娟[1] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2002年第2期55-58,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：林业部重点指南资助项目 (94-0 8-0 2 )

摘　　要：采用 4种质量分数的PEG 60 0 0 ( 1 0 %～ 30 % )对银杏幼苗进行根际渗透胁迫处理。结果表明 ,银杏体内脯氨酸含量、POD活性、膜相对透性及MDA含量对渗透胁迫做出了不同的反应。POD在第 2天出现高峰 ( 1 0 %除外 )后下降 ;脯氨酸含量的显著增加出现 2d之后 (特别是PEG2 0 %～ 30 %强度下 )上升幅度很大 ;膜相对透性增加在脯氨酸含量变化之后。脯氨酸含量与膜相对透性相关显著 (r=0 .97) ,与MDA呈一般的相关。 4种胁迫强度可划分为 3种类型 :1 0 %～1 5 %PEG处理时为轻度胁迫型 ,1 5 %～ 2 0 %处理为中度胁迫型 ,2 0 %～ 30 %处理为严重胁迫型。银杏能较长时间地忍受 2 0 %PEG处理以下的水分胁迫 。The content of free proline,peroxidase(POD)and the relative permeability of plasma membrane(RPM)in seedlings of Ginkgo biloba were studied under different levels of osmotic stress by PEG(6000,10%～30%).The results showed that the POD activity increased in first two days(except 10%),then decreased proline content increased from the second day to the sixth day especially under 20%～30% it increased sharply,while RPM increased greatly from the forth day.In the three indications,the peak of POD activity appeared most early,then proline content and RPM last.There was significant correlation between proline content and RPM( r =0.97)and a general correlation between proline content and MDA.As a result,three categories were classified in the four levels of osmotic stress.Ginkgo seedlings was able to endure the 20%PEG(6 000)level of water stress and would be harmed above that of level.

关 键 词：银杏 幼苗 水分胁迫 生理响应 脯氨酸 膜相对透性 过氧化物酶活性 

分 类 号：S567.19[农业科学—中草药栽培] S792.95[农业科学—作物学]