猫脊髓部分去传入后神经营养素家族表达的时空变化  

The Spatiotemporal Change of Neurotrophin Family and Their mRNA Expression in Spinal Cord of Cats after Partial Rhizotomy

作　　者：周雪[1] 吴良芳[1] 谌宏鸣[1] 王廷华[1] 陈丽华[1] 保天然[1] 廖德阳 

机构地区：[1]四川大学华西基础医学与法医学院组织学研究室,成都610041

出　　处：《华西医科大学学报》2002年第2期165-168,191,共5页Journal of West China University of Medical Sciences

基　　金：国家自然科学基金 (批准号 3980 0 191);四川省科委基金资助

摘　　要：目的　探讨部分去传入后猫脊髓背角 板层内 NGF、BDNF、NT- 3及其 m RNA的时空变化规律。方法　用免疫组化和原位杂交技术检测部分去传入 (切断 L1 ～ L5、L7～ S2 背根 ,保留 L6背根为备用根 ) 3～ 5天、10～ 11天后 ,猫脊髓 L3 、L5、L6或 L7节段背角 板层内三种神经营养素及其 m RNA的表达变化。结果　 1正常猫 NGF及其 m RNA分布于 板层神经元内 ;BDNF分布于神经纤维终末、膨体和神经元内 ,m RNA阴性 ;NT- 3分布于神经元、胶质细胞和少量神经纤维及膨体中 ,m RNA阴性。 2去传入后 3～ 5天 ,NGF及其 m RNA阳性神经元数明显增多 ,NT- 3阳性神经元和胶质细胞数也明显增多 ,而 BDNF含量及阳性膨体数却明显减少 ;10～ 11天时 ,NGF及其 m RNA阳性神经元数仍维持高峰水平 ,NT- 3者虽仍高于正常但已有所下降 ,而 BDNF含量除 L7节段外已恢复到正常 ,但阳性膨体数仍未恢复。 BDNF和 NT- 3的 m RNA仍呈阴性。 3NGF达高峰的时间在 L5、L6节段较早 ,L3 较晚 ;BDNF含量的恢复以 L7最迟 ;NT- 3阳性胶质细胞数量的变化在各节段一致 ,唯 L5、L7节段的阳性神经元数在 10～ 11天时即降致正常水平 ,L3 节段仍维持较高水平。结论　三种因子均参与部分去传入后脊髓的可塑性反应 ,但其发挥作用的早晚及维持时间却不尽相?Objective To investigate the spatiotemporal change rule of NGF,BDNF,NT-3 and their mRNA expression in spinal cord of cats after partial dorsal rhizotomy. Methods Rhizotomy of unilateral L 1~L 5, L 7~S 2 dorsal roots of cats was performed, leaving L 6 as a spared dorsal root. By using ABC immunohistochemistry and in situ hybridization techniques, the dynamic changes of the above three factors and their mRNA in spinal lamina Ⅱ of different segments were analysed. Results ①In normal cat,NGF and it's mRNA were detected in part of neurons; BDNF, in nerve fiber terminals, varicosities and neurons; NT-3, in part of neurons,neuroglias and few nerve fiber terminals and varicosities. The mRNAs of the later two were negative. ②The population of NGF and NGFmRNA positive neurons, NT-3 positive neurons and neuroglias increased significantly 3d-5d after rhizotomy.However,the quantity and density of positive varicosity of BDNF decreased. At 10-11d, the population of NGF and NGF mRNA positive neurons was still on the high level as that at 3-5d, and that of NT-3 began to decrease;the quantity of BDNF recovered to normal except for L 7 segment, but the density of positive varicosity of BDNF did not yet.The mRNAs of BDNF and NT-3 were still negative.③The change of each factor varied with the segments.The highest level time of NGF was earlier in L 5, L 6 than in L 3; the recovery of the quantity of BDNF was the latest in L 7; the change of NT-3 positive neuroglia was the same at each segment,but the number of NT-3 positive neuron in L 5, L 7 returned to normal at 10-11d, and that of L 3 did not. Conclusion The three factors all play roles in spinal plasticity after partial rhizotomy, but they function at different time phase and last different time length.

关 键 词：猫 脊髓 部分去传入 神经营养因子 时空变化 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]