肝细胞膜钙泵与钠泵活性变化在胆红素钙结石形成中的作用  

The Effects of Activities of Ca^(2+)-ATPase and Na^+-K^+-ATPase in Plasma Membranes of Hepatocytes on the Formation of Calcium Bilirubinate Gallstone

作　　者：庄文[1] 肖路加[1] 左凤琼[2] 吴兆锋[2] 吴忠舜[3] 

机构地区：[1]四川大学华西医院普外科,成都610041 [2]四川大学华西基础医学与法医学院生物化学教研室 [3]四川大学华西公共卫生学院病理科

出　　处：《华西医科大学学报》2002年第2期262-264,共3页Journal of West China University of Medical Sciences

基　　金：国家教委博士学科点专项科研基金资助

摘　　要：目的　探讨肝细胞膜钙泵和钠泵活性变化在胆红素钙结石形成中的作用。方法　建立胆红素钙结石兔模型 ,对照组 2 8只 ,胆道不全梗阻组 (BO) 36只 ,胆道不全梗阻加感染组 (BOI) 39只 ,各组再按术后处死时间 (3、7、14和 2 0天 )分组 ,动态测定各组各时相肝细胞膜钙泵、钠泵活性及肝细胞钙含量。结果　 BOI组及 BO组肝细胞钙进行性增多 ,而肝细胞膜钙泵、钠泵活性呈进行性下降 ,与对照组相比差异有显著性 (P<0 .0 1) ,BOI组上述变化较 BO组明显 (P<0 .0 5 )。结论　兔胆红素钙结石形成过程中存在肝细胞膜钙泵和钠泵活性渐进性下降。钙泵、钠泵活性下降引起肝细胞钙超负荷 。Objective To investigate the effects of activities of Ca 2+-ATPase and Na +-K +-ATPase in plasma membranes of hepatocytes on the formation of calcium bilirubinate gallstone. Methods The rabbit models for studying calcium bilirubinate gallstone were used.One hundred and three rabbits were randomly divided into the control (sham operation) group (Con, n=28), the simple biliary obstruction group (BO, n=36), and the biliary obstruction and infection group (BOI, n=39). The activities of Ca 2+-ATPase and Na +-K +-ATPase in plasma membranes of hepatocytes and the intracellular calcium content were measured on the 3rd,7th,14th and 20th days after operation. Results The activities of Ca 2+-ATPase and Na +-K +-ATPase decreased remarkably in all phases of BOI and BO groups as compared with those of Con group (P<0.01). The over-loaded intracellular calcium was found in both BOI and BO groups. The above-mentioned changes wer more significant in BOI group than in BO group (P<0.05). Conclusion The progressive decrease of the activities of Ca 2+-ATPase and Na +-K +-ATPase is in close relationship with the continuous increase of intracellular calcium content during the formation of calcium bilirubinate gallstone in rabbit models. Infection can aggravate those changes and further the formation of stone.

关 键 词：肝细胞膜 钙泵 钠泵 活性 胆红素钙结石 兔模型 

分 类 号：R657.4[医药卫生—外科学]