IL—12对干扰素α治疗的慢性乙型肝炎患者外周血单个核细胞产生细胞因子的影响  

Effect of IL-12 on IFN_γ and IL-10 produced by peripheral blood mononuclear cells in patients with chronic hepatitis B virus infection during IFN α treatment

作　　者：王少扬[1] 林玉梅[1] 马卫闽[1] 张宝华[2] 戚少然[2] 兰凤华[2] 

机构地区：[1]南京军区福州总医院传染科,福州350003 [2]南京军区福州总医院实验科

出　　处：《中华肝脏病杂志》2002年第2期116-119,共4页Chinese Journal of Hepatology

摘　　要：目的 探讨干扰素α治疗慢性乙型肝炎过程中ＩＬ－１２对其外周血单个核细胞（ＰＢＭＣ）产生 ＩＦＮ γ和 ＩＬ—１０的动态影响。方法 用 ＥＬＩＳＡ法分别检测 ２０例慢性乙型肝炎患者在干扰素α２ｂ治疗前、治疗 ３个月、治疗 ６个月时 ＰＢＭＣ在 ＰＨＡ（１００μｇ／ｍｌ）、ＨＢｃＡｇ（１μｇ／ｍｌ）、ＨＢｅＡｇ（１μｇ／ｍｌ）单独或联合 ＩＬ—１２（１０ｎｇ／ｍｌ）体外培养４８ｈ后培养上清液ＩＮＦγ和ＩＬ—１０的水平。结累 １２例干扰素应答患者联合ＩＬ—１２诱导，同一诱导组在治疗 ３个月和 ６个月同治疗前比较，ＩＦＮ γ水平明显增高（Ｐ＜０．０１），同一治疗阶段，单独抗原诱导和联合 ＩＬ—１２诱导相比，联台诱导组 ＩＦＮ γ水平明显增高（Ｐ＜０．０１），治疗 ３个月和 ６个月时，ＩＬ一１２对ＩＬ－１０ 的产生表现出明显的抑制作用（ＨＢｃＡｇ诱导组例外）。８例干扰素无应答患者联合ＩＬ—１２诱导，同一诱导组在治疗３个月和６个月同治疗前相比，ＩＦＮγ水平明显增高（Ｐ＜０．０１），同一治疗阶段，单独抗原诱导和联合ＩＬ－１２诱导相比，联合诱导组ＩＦＮ γ水平明显增高，并随治疗时间的延长，协同效应更为明显。结论 在干扰素治疗过程中，ＩＬ—１２对乙?Objective To investigate the effect of IL-12 on IFN γ and IL- 10 production of peripheral blood mono- nuclear cells (PBMC) in chronic hepatitis B virus infection patients during IFN α treatment. Methods Before and after IFN α treatment of 3 months and 6 months, PBMC of 20 patients with chronic hepatitis B virus infection were collected and cultured in vitro in the culture fluid containing PHA (100 μ g/ml), HBcAg (1 μg/ml), or HBeAg (1μg/ml) for 48 h under the condition of the presence or absence of IL-12 (10 ng/ml). Then the levels of IFN γ and IL-10 were determined by ELISA. Results There were 12 responders and 8 nonresponders to IFN α treatment. In the responders, the enhancing ef- fect of IL-12 on IFN γ production was significantly greater after IFN α treatment than before the treatment. The production of IL-10 was suppressed in the presence of IL-12 after 3 months and 6 months of IFN α treatment. In the same treatment time, the level of IFN γ in the presence of IL-12 was significantly higher than that in the absence of IL-12. To the nonresponders, the enhancing effect of IL-12 on IFN γ production was also significantly increased after IFN-α treatment. Moreover, in the same treatment time, the level of IFN γ in the presence of IL-12 was significantly higher than that in the absence of IL-12. Conclusions The enhancing effect of IL-12 on IFN γ production of PBMC in patients with chronic hepatitis B virus infection is increased during IFN α treatment. IFN α and IL-12 may enhance the effcacy for the treatment of chronic hepatitis B virus infection.

关 键 词：慢性乙型肝炎 外周血单个核细胞 影响 干扰素Α 白细胞介素12 细胞因子 

分 类 号：R512.62[医药卫生—内科学]