编码日本血吸虫抱雌沟蛋白及肌动蛋白的基因性别间的表达差异性  

SEXUAL DIFFERENCE IN EXPRESSION OF THE GYNECOPHORAL CANAL PROTEIN GENE AND THE ACTIN GENE OF SCHISTOSOMA JAPONICUM (CHINESE STRAIN)

作　　者：朱建国[1] 林矫矫[1] 杨冠珍[2] 倪振亚[1] 陈兆国[1] 吴祥甫[2] 周元聪[2] 蔡幼民[1] 

机构地区：[1]中国农业科学院上海家畜寄生虫病研究所 [2]中国科学院上海生物化学研究所,上海200031

出　　处：《动物学报》2002年第2期240-244,共5页ACTA ZOOLOGICA SINICA

基　　金：国家高技术"86 3"基金 (No .10 2 0 7 0 4 0 3)~~

摘　　要：研究了所克隆的日本血吸虫两个基因的性别间的表达差异性。分别将所克隆到的编码日本血吸虫抱雌沟蛋白的cDNA (SjGCP1)和肌动蛋白的cDNA (SjAct)制成DNA探针 ,利用Southernblotting ,Northernblotting和斑点印迹法对其相应基因性别间的表达差异性进行了研究。结果显示 ,日本血吸虫抱雌沟蛋白的基因在雄虫中转录而雌虫中不转录 ,血吸虫肌动蛋白基因在雄虫中转录量高于雌虫 ,表现出转录量的差异。证明这两个基因转录具有性别间的表达差异性。A 868 bp cDNA fragment, named SjGCP1, encoding part of gynecophoral canal protein, and a 1 131 bp cDNA fragment,named SjAct, encoding actin, were amplified with RT PCR from mRNA of adult Schistosoma japonicum (Chinese strain). Sexual differences of the corresponding genes were studied by Southern blotting, Northern blotting and Dot blotting with α P 32 labeled probes synthetized with the two fragments using Nick Translation Kit respectively. Southern blotting showed that both SjAct and SjGCP1 are all positive in male and female samples while the result of Northern blotting indicated that SjAct is positive in both sexes and SjGCP1 positive only in male. Additionally, although SjAct was shown to be transcribed in both male and female, the amount of RNA transcribed is much more in male than that in female. This led us to conclude that SjGCP is only expressed in male and SjAct is expressed more favorably in male than in female.

关 键 词：日本血吸虫 抱雌沟蛋白 肌动蛋白 基因表达差异性 性别 

分 类 号：S852.735[农业科学—基础兽医学]