杀虫剂亚致死剂量对小菜蛾羧酸酯酶的影响  

Effect of Sublethal Concentration of Insecticides on theCarboxylesterase in Diamondback Moth, Plutella xylostella (L.)

作　　者：夏冰[1] 石泰[1] 梁沛[1] 高希武[1] 

机构地区：[1]中国农业大学植物保护学院

出　　处：《农药学学报》2002年第1期23-27,共5页Chinese Journal of Pesticide Science

基　　金：国家重大基础研究 973(G2 0 0 0 0 16 2 0 7) ;国家自然科学基金资助项目 (2 9832 0 2 5和 39970 4 96 )

摘　　要：就阿维菌素和高效氯氰菊酯亚致死剂量对小菜蛾 Plutella xylostella L inneae羧酸酯酶(Car E)活性的影响进行了研究。用亚致死剂量的阿维菌素和高效氯氰菊酯分别处理阿维菌素敏感和抗性小菜蛾 ,使敏感品系 Car E比活力上升 ,抗性品系 Car E比活力下降。酶动力学研究表明 ,两种药剂亚致死剂量处理对小菜蛾 Car E与底物 α- NA亲和力的影响存在差异。在敏感品系中 ,阿维菌素和高效氯氰菊酯处理前后 Car E的 Km 值无明显变化 ;抗性品系中 ,用阿维菌素亚致死剂量处理后 ,小菜蛾体内 Car E的 Km 值显著高于对照 ,即其对 α- NA的亲和力比对照组明显降低 ,高效氯氰菊酯处理组 Car E的 Km 值与对照相比无明显变化。The sublethal effect of abamectin (AVM) and beta cypermethrin(CPM) on the carboxylesterase (CarE) in diamondback moth (DBM), Plutella xylostella (L.) was investigated. The results showed that the specific activity of CarE in DBM increased in abamectin susceptible strain(XH S)and decreased in abamectin resistant strain(AV R) after the treatment with sublethal concentration of AVM and CPM. The effect of AVM and CPM sublethal concentration on the affinity of CarE to α or β naphthyl acetate is varied. After treated with AVM and CPM, the K m value of CarE in XH S strain showed no distinct difference from that of the control, In the AV R strain, the K m value of CarE after treated with AVM is significantly higher than that of the control but no distinct change after treatment with CPM.

关 键 词：杀虫剂 亚致死剂量 小菜蛾 羧酸酯酶 影响 阿维菌素 高效氯氰菊酯 

分 类 号：S482.3[农业科学—农药学] S433.4[农业科学—植物保护]