抗白一号诱导白血病K562细胞凋亡作用的研究  

Study of Traditional Chinese Medicine Recipe in inducing leukemic K562 cells apoptosis

作　　者：孙宏丹[1] 孟秀香[1] 贾莉[1] 宋振岚[1] 

机构地区：[1]大连医科大学检验医学院临床血液教研室,大连116027

出　　处：《中药药理与临床》2002年第2期34-35,共2页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的 :研究抗白一号对白血病细胞株K5 6 2细胞的影响 ,并探讨其机制。方法 :采用MTT法检测细胞毒作用 ,利用Wright Gimesa染色法、Hoechst33342荧光染色等手段观察凋亡细胞的形态结构变化 ,应用透射电镜技术和流式细胞分光光度术进行凋亡定性定量观察 ,并探讨凋亡抑制基因bcl 2蛋白表达 ,应用原位末端标记检测DNA断裂。结果 :抗白一号对细胞具有明显的生长抑制作用。在其作用下细胞呈现典型的凋亡形态学变化。流式细胞仪分析表明抗白一号能使K5 6 2细胞发生凋亡 ,凋亡率呈现对浓度和时间的依赖性 ,并发现抗白一号使K 5 6 2细胞bcl 2基因的表达下调。结论 :抗白一号能诱导K5 6 2细胞发生凋亡 ,其作用机制与bcl 2基因表达的下调有关。Objective: To study the effect and mechanism of TCM Recipe (TCMR) in inducing K562 cell line apoptosis. Methods: MTT, light microscopy, transmission electromicroscopy (TEM) and flow cytometry were used. Results: The results showed that TCM Recipe could inhibit the proliferation of K562 cell line. The effect of TCMR on the growth of K562 cell line was evaluated by MTT assay and the IC_ 50 values were 0.874±0.076mg/ml. The apoptosis of K562 cell line was conformed by flow cytometry and characteristic ultrastructure alterations. The results also demonstrated that inhibitory effects of TCMR were associated with the high percentage of hypodiploid cells. The expression of bcl-2 protein decreased in a dose and time-dependent manner in K562 cell line. Conclusion: It is suggested that induction of cell apoptosis can be one of the mechanisms of the therapeutic effect of the TCM Recipe. Moreover, this apoptosis induction occurs through the modulation of bcl-2.

关 键 词：抗白一号 细胞凋亡 K562细胞 BCL-2基因 白血病 作用机制 

分 类 号：R273[医药卫生—中西医结合]