Cystatin-ELISA法诊断日本血吸虫病的研究  

CYSTATIN-ELISA FOR IMMUNODIAGNOSIS OF HUMAN SCHISTOSOMIASIS

作　　者：黄跃龙[1] 易新元[1] 曾宪芳[1] 张顺科[1] 李忠杰[1] 袁仕善[1] 周东明[1] 王庆林[1] 

机构地区：[1]中南大学湘雅医学院寄生虫学教研室,长沙410078

出　　处：《中国血吸虫病防治杂志》2002年第2期115-118,共4页Chinese Journal of Schistosomiasis Control

摘　　要：目的　建立一种敏感、高特异性的血吸虫病诊断方法。方法　应用 Cystatin捕获血吸虫吐出物中的半胱氨酸蛋白酶 ,再进行 EL ISA试验检测日本血吸虫 (Sj)病人血清中相应的特异抗体。结果　检测 12 0例慢性血吸虫病人血清 ,84例非疫区正常人血清和 36例华支睾吸虫病人血清 ,Cys-tatin- EL ISA检测 Sj病人阳性率为 98.4 % ,与正常人及华支睾吸虫病人无交叉反应。其敏感性(98.4 % )与 SEA- EL ISA法的敏感性 (93.4 % )比较无明显区别 (P>0 .0 5 ) ,但特异性显著高于后者(P<0 .0 5 )。 Cystatin- EL ISA敏感性和特异性亦均高于 AWA- EL ISA和 ES- EL ISA。结论　 Cys-tatin- EL ISA敏感性高 ,特异性强 ,是一种有效的诊断血吸虫病的方法。Objective To develop an accurate and highly specific assay for diagnosis of schistosomiasis. Methods The ELISA was performed using cystatin to capture cysteine proteinases in the Schistosoma japonicum (Sj) excretion secretion(ES) and to detect specific antibodies to cysteine proteinases in the sera from patients with chronic schistosomiasis japonica. Results One hundred and twenty endemic sera from patients with chronic schistosomiasis, 84 sera from normal population in non endemic area and 36 sera from patients wtih clonorchiasis were tested. The Cystatin ELISA showed the sensitivity (98 4%) similar to that of the SEA ELISA (93 4%) (P>0 05), and the specificity higher than that of the SEA ELISA (P<0 05). Conclusion Cystatin ELISA has good sensitivity and high specificity and is useful for the immunodiagnosis of schistosomiasis.

关 键 词：血吸虫病 ES Cystatin-ELISA法 诊断 抗体 日本血吸虫 

分 类 号：R532.210.4[医药卫生—内科学]