VEGF在激光TMLR中促心肌血管生成的实验研究  

EXPERIMENTAL STUDY ON THE IMPROVEMENT OF ANGIOGENESIS AND REVASCULARIZATION BY VEGF IN TMLR MODELS

作　　者：张晓东[1] 曹润武[2] 李若凡[1] 程效东[1] 王冬[1] 曲彦明[1] 张平[1] 徐达传[3] 

机构地区：[1]中国医学科学院基础医学研究所中国协和医科大学基础医学院人体解剖学与组织学胚胎学系,北京100005 [2]中国人民解放军第306医院心胸外科,北京100101 [3]第一军医大学解剖学教研室,广州510515

出　　处：《解剖学报》2002年第2期204-207,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 (3 9870 3 85 )

摘　　要：目的　从形态学角度观察血管内皮细胞生长因子 (vascularendothelialgrowthfactor,VEGF)在激光透室壁性心肌血管重建术 (transmyocardiallaserrevascularization ,TMLR)中 ,促TMLR孔道内血管生成的作用 ,探讨优化TM LR的可行性方案。　方法　采用高能CO2 激光照射家兔左室前壁 ,形成透室壁性激光心肌孔道。实验组TMLR孔道内注射VEGF ;对照组TMLR孔道内注射相同剂量的生理盐水。动物于术后不同时间处死 ,利用组织切片染色法 ,对照观察实验组和对照组的TMLR孔道内血管生成状况。　结果　应用VEGF实验组和未应用VEGF对照组的TMLR孔道内血管生成的密度 /数量具有显著性差异 ,P <0 0 0 1。Objective To research the effects of vascular endothelial growth factor(VEGF) on angiogenesis and revascularization in view of morphology. Methods Transmyocardial channels were made by CO 2 laser in vivo on left anterior ventricular wall of the rabbits (transmyocardial laser revascularization, TMLR). The control group was injected with physiological saline in channels after TMLR, but the experimental group was injected with VEGF. The animals of two groups were sacrificed at intervals of 1,2,4 and 6 8 weeks after TMLR. The effects of angiogenesis and revascularization were observed and the new blood vessels in channels of two groups were measured under microscope. Results Histological analysis demonstrated a high significant difference between the experiment and control groups in the density of new vessels(NV), P <0 001.Conclusion The results confirm that the effects of VEGF on angiogenesis and revascularization in TMLR channels are significant.\;[

关 键 词：血管内皮细胞生长因子 透室壁性激光心肌血管重建术 血管生成 激光孔道 动物实验 

分 类 号：R322.11[医药卫生—人体解剖和组织胚胎学] R654.2[医药卫生—基础医学]