地塞米松对小鼠血栓形成的影响  

Effect of dexamethasone on thrombosis in mice

作　　者：沙建慧[1] 黄兰玲[2] 夏文春 

机构地区：[1]第四军医大学吉林军医学院生理教研室,吉林吉林132013 [2]第四军医大学吉林军医学院附属医院检验科 [3]吉林市医院血液科

出　　处：《吉林大学学报（医学版）》2002年第2期216-218,共3页Journal of Jilin University：Medicine Edition

摘　　要：目的 :研究不同剂量地塞米松对小鼠体内血栓形成的影响。方法 :用角叉菜胶诱发小鼠尾部血栓的模型 ,测量尾部血栓形成长度百分数、凝血酶原时间 ( PT)、红细胞数 ( RBC)、红细胞平均血红蛋白量 ( MCH)、红细胞压积 ( HCT)和血小板数 ( PLT) ,用发色底物法测定血浆组织型纤溶酶原激活物 ( t- PA)及纤溶酶原激活物抑制物 ( PAI)活性。结果 :高剂量地塞米松组与模型对照 组相比 ,尾部血栓形成长度百分数增加 ,RBC、MCH、HCT和 PAI活性也有不同程度的提高 ( P<0 .0 5或 P<0 .0 1 ) ;小剂量地塞米松组与模型对照 组相比 ,尾部血栓形成长度百分数降低、PT延长、t- PA活性增加 ( P<0 .0 5 )。结论 :地塞米松在血栓形成机制中具有双重作用 ,即高剂量地塞米松使血栓形成加重主要与提高 Hb、RBC使血液粘度比提高和 PAI释放增加使纤溶系统被抑制有关 ;低剂量地塞米松具有抑制血栓形成作用 ,其作用机理可能与外源性凝血因子减少以及 t-Objective: To investigate the effect of different dose of dexamethasone on thrombosis model of mice. Methods: Tail thrombosis was induced by carrageenan. The percentage of thrombosis length in tail, protime(PT), red blood cell number (RBC), mean cell hemoglobin (MCH), haematocrit (HCT) and platelet count (PLT) were detected. The plasma t PA and PAI activities were detected by chromogennic substrate analysis. Results: Compared with the thrombosis control groupⅠ, the percentage of thrombosis′ length in tail, RBC,MCH,HCT and PAI in the higher dose group were significantly increased ( P<0 05 or P <0 01). In comparision with the thrombosis control groupⅡ, the percent of thrombosis length in tail decreased, PT lengthened and t PA activity were increased ( P <0 05) . Conclusion: Dexamethasone had double mechanism on the thrombosis. The fact that higher dose of dexamethasone promoted thrombosis mainly was due to the increase of blood viscosity because of increased Hb and RBC and the refrained fibrinolysis system caused by the increase of PAI; The lower dose of dexamethasone had the effect of refraining thrombosis. The mechanism may be associated with the strengthened activity of fibrinolysis system from decreased exogenous coagulation factors and increased t PA.

关 键 词：地塞米松 血栓形成 动物实验 作用机理 激素 

分 类 号：R977.11[医药卫生—药品] R364.15[医药卫生—药学]