抗人甲状腺素单克隆抗体的制备及其初步的临床应用  

The Preparation, Characteristics and the Preliminary Clinical Application of Antihuman Thyroxine Monoclonal Antibodies

作　　者：朱建衡[1] 吴复平[1] 刘璐[1] 蒋清[2] 高翼之[2] 单祥年[2] 

机构地区：[1]南京铁道医学院附属医院同位素科,210009 [2]南京铁道医学院生物教研室

出　　处：《铁道医学》1991年第3期134-136,共3页Railway Medical Journal

摘　　要：本文用甲状腺素(T_4)甲酯—BSA抗原免疫BALB/C小鼠,获得4株分泌抗人甲状腺素单克隆抗体的杂交瘤细胞系。亚类鉴定均属IgG_1型。将单克隆抗体应用在放射免疫分析(RIA)中,取代多克隆抗体,证实其在甲状腺疾病诊断中,甲状腺机能亢进的诊断符合率高于多克隆抗体,显示其特异性强、灵敏度高、亲和力好(亲和常数K=1.26x10^(10)L/M)、反应平衡时间短等优点。Thyroxine(T_4)was firstly methylesterized and coupled to bovine serum albumin (BSA) to form T_4-methylesterBSA. Then BALB/C mice were immunizcd. The spleen cells of mice were fused with mouse FO myeloma cells. Then the monoclonal antibodies were prepared by selective antibody-producing clones. Subclass determination showed that these MoAbs belong to IgG1. The McAbs has the advantages of high sensitivity, specifity and affinity (K=1.26×10^(10)L/M). Using these MoAbs in T_4 radioimmunassay, the time of reaction to equilibrium was only 15-30 minutes. The preliminary clinical application showed that the diagnostic agreement rate to hyperthyroidism of using MoAbs was higher than that of using rabhit antiserum, the former was 92.1%, while the later was 84.8%. The diagnosis agreement rate to hypothroidism was 100％. The lowest determinable value was 1.1μg/L.

关 键 词：甲状腺素 单克隆抗体 制备 

分 类 号：R392－33[医药卫生—免疫学]