HPLC法测定四环素可的松眼膏中醋酸可的松的含量  

HPLC DETERMINATION OF CORTISONE ACETATE IN TETRACYCLINE CORTISONE EYE OINTMENT

作　　者：陈伟[1] 仇士林[1] 

机构地区：[1]上海市药品检验所,上海200233

出　　处：《中国药品标准》2002年第2期34-36,共3页Drug Standards of China

摘　　要：目的:建立HPLC法测定四环素可的松眼膏中醋酸可的松的含量。方法:醋酸可的松经甲醇提取后,以Inertsil ODS—3为分析柱,甲醇-水(70:30)为流动相,240nm为检测波长。结果:醋酸可的松平均回收率为100.0％,RSD=0.4％(n=6)。结论:本方法简便,快速,结果准确。To establish a method for determination of cortisone acetate in Tetracycline Cortisone Eye Ointment.Method: Cortisone acetate was extracted with methanol and determined by HPLC using Inertsil ODS—3 as column,methanol—water(70:30)as the mobile phase.and detecting at 240nm.Results:The average recovery was 100.0%,RSD was 0.4%(n=6).Conclusion:The method is simple,quick and accurate.

关 键 词：四环素可的松眼膏 醋酸可的松 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学] R988.1