传染性法氏囊病病毒变异E株感染鸡细胞凋亡的研究  

Studies on Apoptosis in Chickens Infected with Variant Serotype-1 Isolate E of Infectious Bursal Disease Virus

作　　者：陈明勇[1] 高齐瑜[1] 杨汉春[1] 乔健[1] 

机构地区：[1]中国农业大学动物医学院,北京100094

出　　处：《畜牧兽医学报》2002年第2期180-184,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：研究了传染性法氏囊病病毒（IBDV）变异E株人工感染28日龄SPF雏鸡后鸡法氏囊淋巴细胞的凋亡情况。电镜观察和DNA电泳分析结果表明,IBDV感染后12～48h,雏鸡法氏囊淋巴细胞出现典型细胞凋亡的形态学特征和生化特征;经流式细胞计检测和荧光染色观察,统计学分析表明,IBDV感染后24～48h,雏鸡法氏囊淋巴细胞凋亡数量显著增加（P<0.05或P<0.01）。试验结果揭示IBDV变异E株人工感染可以诱导雏鸡法氏囊淋巴细胞凋亡。Apoptosis of the bursal lymphocytes of chickens infected with variant serotype-1 isolate E of infectious bursal disease virus(IBDV) at 28-day-old was studied. The IBDV-infected bursal lymphocytes exhibited the morphological and biochemical features of apoptosis at 12 -48 h after infection using transimission electron microscope and agarose gel electrophoresis of cellular DNA. Statistical analyses by flow cytometry and staining with fluorescent DNA-binding dyes indicated that the number of apoptotic cells in the bursal lymphocytes increased signficantly by 24-48 h post infection(P<0.05 or 0.01). The results revealed that the bursal lymphocytes of chickens infected artifically with IBDV was induced to display apoptosis.

关 键 词：传染性法氏囊病病毒 变异E株 细胞凋亡 SPF鸡 免疫抑制 

分 类 号：S858.315.3[农业科学—临床兽医学]