东乡野生稻等位酶位点遗传多样性的初步研究  

A Preliminary Study on the Genetic Diversity of Allozymic Loci of Dongxiang Wild Rice (Oryza rufipogon Griff.)

作　　者：黄英金[1] 李桂花[1] 陈大洲 石庆华[1] 肖玉峰[1] 王锋尖[1] 余丽琴 肖叶青 

机构地区：[1]江西农业大学农学院,江西南昌330045 [2]江西省农科院水稻研究所,江西南昌330200

出　　处：《江西农业大学学报》2002年第1期1-5,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金重点项目 (3996 90 0 1)

摘　　要：采用聚丙烯酰胺凝胶电泳技术 ,对东乡野生稻 9个天然小群体进行了 15个等位酶位点的遗传多样性分析。结果表明 ,东乡野生稻具有较高的遗传变异水平 ,9个小群体的多态位点百分率 (P)为 2 8.2 % ,等位基因平均数 (A)为 1.30 6 ,预期杂合度 (He)为 0 .2 34;小群体间的分化程度不高 ,基因分化系数 (Gst)为 0 .0 12 ,等位酶测定的总变异中 ,1.2 %来自小群体间 ,其余的变异 (98.8% )来自小群体内。Genetic diversity of 15 allozymic loci in nine seperate clusters of Dongxiang wild rice (Oryza rufipogon Griff.) was estimated by vertical slab polyacrylamide gel electrophoresis. The results were as follows: The level of genetic variability in the population of Dongxiang wild rice was relatively high. The percentage of polymorphic loci (P) was 28.2%. The average number of alleles per loci (A) was 1.306. The average expected heterozygosity (He) was 0.234, similar to reports for other conifers, the differentiation level of Dongxiang wild rice was relatively low. The coefficient of gene differentiation (Gst) was 0.012, 1.2% of the allozyme variability was attributed to the differentiation among the clusters while the remaining 98.8% resided within the different clusters. The results suggested strategies for the conservation of Dongxiang wild rice.

关 键 词：东乡野生稻 普通野生稻 等位酶 遗传多样性 聚丙烯酰胺凝胶电泳 

分 类 号：S511.903.2[农业科学—作物学]