东方蜜蜂DNA随机扩增多态及其遗传分化研究  

Genetic divergences of apis cerana from Yunnan of China and Malaysia were estimated by rapd analysis

作　　者：董霞[1] 张炫[1] 史宪伟[2] 汪建明[1] 陈永久[2] 张亚平[2] 

机构地区：[1]云南农业大学东方蜜蜂研究所,昆明650201 [2]中国科学院昆明动物所细胞与分子进化开放实验室,昆明650223

出　　处：《中国养蜂》2002年第2期9-10,18,共3页Apiculture of China

基　　金：云南省自然科学基金资助项目 (项目编号 :97C0 0 6Q)

摘　　要：本研究对云南及马来西亚的 37个东方蜜蜂样本进行随机扩增多态DNA分析 ,从 2 0个引物中筛选出 1 1个引物 ,其中 9个引物扩增出多态带。共检测到 6 6条扩增片段 ,其中 5 6条为多态带。用UPGMA聚类方法构建的分子系统树显示 ,云南的样本、马来西亚的样本各自分别聚在一起 ,说明两个样本间遗传差异较大 ,群体之间存在着遗传分化。但就云南的 32个个体而言 ,虽然聚类图中大多数采自同一地区的样本聚在一起 ,但也存在一定交叉 ,提示云南地理群体间近期可能存在一定的基因流。In this study,random amplified polymorphic DNA(RAPD) analysis was used to estimate genetic diversity and relationship in 37 Apis cerana workers individuals from Yunnan of China and from Malaysia.20 primers were used among which 9 generated polymorphic loci.A total of 66 bands were scored,of which 56(84.85%)were polymorphism.The genetic distance matrix was obtained by proportion of shared fragments.Dendrogram was generated by UPGMA.There were genetic divergences among Apis cerana from Yunnan province of China and from Malaysia.;

关 键 词：东方蜜蜂 RAPD 遗传分化 DNA随机扩增多态 

分 类 号：S891[农业科学—特种经济动物饲养]