玉米自交系7922幼胚愈伤组织诱导条件的优化  

Optimum Condtions of Callus Induction in Maize Inbred Line 7922 from Immature Embryos

作　　者：王雷[1] 王丕武[1] 张君[1] 武丽敏[1] 刘金华[1] 

机构地区：[1]吉林农业大学生物中心,吉林长春130118

出　　处：《玉米科学》2002年第1期18-20,共3页Journal of Maize Sciences

基　　金：国家转基因植物中试与产业化基地建设"专项基金(J99_B_0 0 1)

摘　　要：以玉米自交系 792 2的幼胚为外植体 ,全面探讨了影响其愈伤组织诱导的主要因素 ,如接种方式、4℃保存时间、碳源种类及其浓度、培养基成分、AgNO3等。结果表明 :采用盾片侧向上的方式 ,取 4℃保存时间不超过 7天的幼胚 ,以 8114、MB、MS、N6为基本培养基 ,蔗糖浓度为 4 0g/L或用 2 0g/L的蔗糖 + 10g/L的葡萄糖为较适宜的诱导条件。Factors influencing the callus induction of immatue embryos in maize inbred line 7922 such as ways of inoculation of immature embryos,duration of 4℃treatment,culture medium,carbon source and their concentration,were studied.The results showed that the better conditions of callus induction were the way of inoclation using scutella up,dureation of 4℃ treatment being lower than 7 days,8114,MB,MS,N6 being used as culture medium,concentration of sucrose being 4 g/L or 20 g/L glucose.The effect of AgNO 3 was also studied.The result showed that AgNO 3 had positive effect on callus induction.

关 键 词：玉米 自交系 愈伤组织 诱导条件 优化 幼胚 组织培养 

分 类 号：S513.035.3[农业科学—作物学]