细粒棘球绦虫cDNA克隆EgA31在大肠埃希氏菌M15中的表达及重组蛋白的纯化和复性  

Expression of EgA31 cDNA of Echinococcus granulosus in Escherichia coli M15 and Purification and Renaturation of the Recombinant Protein

作　　者：傅玉才[1] 王进成[2] 张壮志[2] 李雄[3] Anne Francoise Petavy Georges Bosquet 

机构地区：[1]汕头大学医学院,广东汕头515031 [2]新疆畜牧科学院兽医研究所,新疆乌鲁木齐830000 [3]新疆地方病防治研究所,新疆乌鲁木齐830002 [4]法国里昂第一大学,法国里昂696373

出　　处：《中国兽医科技》2002年第1期5-7,共3页Chinese Journal of Veterinary Science and Technology

基　　金：国家自然科学基金资助项目 (39360 0 74)

摘　　要：为了获得细粒棘球绦虫 66ku抗原的重组蛋白和抗血清 ,用该抗原的cDNA克隆EgA3 1构建了pQE3 0EgA3 1重组质粒 ,在大肠埃希氏菌M15中表达 ,经亲和层析获得纯度较高的EgA3 1重组蛋白 ;用经复性处理的重组蛋白免疫动物 ,获得EgA3 1重组蛋白抗血清。本文就重组蛋白的稳定性和在复性处理中遇到的问题进行了讨论。In order to prepare the recombinant protein of EgA31 cDNA representing the 66 ku antigen gene of Echinococcus granulosus and the antiserum, the recombinant plasmid pQE31 EgA31 was constructed and expressed in Escherichia coli M15. The recombinant protein was purified with affinity chromatography by means of using Ni NTA agarose. An anti serum against the recombinant protein was prepared throrgh animals being immunized with the recombinant protein which was purfied. The paper also discussed the stablity and the renaturation of the recombinant protein.

关 键 词：细粒棘球绦虫 抗原 重组蛋白 纯化 复性 cDNA克隆EgA31 大肠埃希氏菌M15 基因表达 

分 类 号：S852.734[农业科学—基础兽医学]