信号肽和辅助性T细胞表位增强HBV核心抗原DNA疫苗诱导的免疫应答  

Enhancement of Immune Responses of Hepatitis B Virus Core DNA Vaccine by a Signal Peptide and a Universal Helper T Lymphocyte Epitope

作　　者：赵平[1] 赵兰娟[1] 曹洁[1] 洪海燕[1] 戚中田[1] 

机构地区：[1]第二军医大学微生物学教研室,上海200433

出　　处：《生物化学与生物物理学报》2002年第3期341-346,共6页

基　　金：国家自然科学基金资助项目 (No .39980 0 38);上海科技发展基金资助项目 (No .0 0 43192 0 6 )~~

摘　　要：为增强HBVDNA疫苗的免疫效率 ,于HBV核心抗原 (HBcAg)基因 5′末端引入人IL 2信号肽和一个通用型辅助性T淋巴细胞表位基因 ,并构建成DNA疫苗 ,转染COS7细胞后经ELISA检测出分泌型HBcAg。通过肌肉注射途径分别将这种DNA疫苗和编码天然HBcAg的DNA疫苗免疫BALB/c小鼠 ,检测小鼠的血清抗体、T细胞增殖和细胞毒性T淋巴细胞反应 ,结果表明前者诱导细胞和体液免疫应答的强度均明显超过后者 ,且更趋向于T辅助细胞 1(Th1)型免疫应答 。The human interleukin-2 signal peptide and a potent universal helper T lymphocyte epitope PADRE were spliced to the 5′ terminus of hepatitis B virus core (HBcAg) gene. The modified HBcAg gene was used to construct a DNA vaccine. After the resulted DNA vaccine construct was transfected into COS7 cells, secreted HBcAg was detected in the supernatant by ELISA. BALB/c mice were vaccinated intramuscularly with the modified HBcAg DNA vaccine and the wild-type one. Serum antibodies, T lymphocyte proliferative response and cytotoxic T lymphocyte response of the immunized mice were measured. The results showed that the modified DNA construct induced cellular and humoral immune responses much stronger in vivo than the natural one did, indicating the potential value as a therapeutic vaccine for treatment of chronic hepatitis B.

关 键 词：信号肽 辅助性T细胞表位 HBV核心抗原 DNA疫苗 诱导 免疫应答 

分 类 号：R392[医药卫生—免疫学]