小尾寒羊4个微卫星座位的克隆及序列分析  

Cloning and Sequencing of Four Microsatellite Loci in Small Tail Han Sheep

作　　者：储明星[1] 王吉振[2] 王爱国[2] 李宁[3] 傅金恋[2] 

机构地区：[1]中国农业科学院畜牧研究所,北京100094 [2]中国农业大学动物科技学院,北京100094 [3]中国农业大学农业生物技术国家重点实验室,北京100094

出　　处：《Acta Genetica Sinica》2002年第5期402-405,共4页

基　　金：国家自然科学基金农业倾斜项目 (批准号 :3 9770 5 41)资助~~

摘　　要：小尾寒羊是我国优良的地方绵羊品种 ,具有极高的繁殖力 ,平均每胎产羔 2 6只。利用与Booroola绵羊高繁殖力主效基因 FecB连锁的 4个微卫星座位 OarAE10 1、OarHH35、BM14 3和BMS2 5 0 8对小尾寒羊、多赛特羊、多赛特公羊×小尾寒羊母羊杂一代羔羊 3个绵羊群体 15 9只绵羊进行了遗传检测 ,证实了微卫星DNA的共显性遗传特性。用非变性 (中性 )聚丙烯酰胺凝胶电泳检测微卫星的PCR扩增产物。对小尾寒羊 4个微卫星座位 6个克隆的PCR扩增片段测序获得的序列已被GenBank接受 ,登录号分别为AF394 4 4 5、AF394 4 4 6、AF394 4 4 7、AF394 4 4 8、AF394 4 4 9、AF394 4 5 0。本研究中小尾寒羊微卫星OarAE10 1的测序结果与GenBank登录的绵羊OarAE10 1序列的同源性为 98% ,小尾寒羊微卫星OarHH35的测序结果与GenBank登录的绵羊OarHH35序列的同源性为 99% ,小尾寒羊微卫星BM14 3的测序结果与GenBank登录的牛BM14 3序列的同源性为 95 % ,小尾寒羊微卫星BMS2 5 0 8的测序结果与GenBank登录的牛BMS2 5 0 8序列的同源性为 95 %。测得的小尾寒羊微卫星OarAE10 1、OarHH35、BM14 3和BMS2 5 0 8均为完全的微卫星 (TG) n。Small Tail Han sheep is an excellent local sheep breed in China. Small Tail Han sheep has significant characteristics of high prolificacy. The lambing percentage averaged 260 percent in Small Tail Han sheep. The genetic detection of 4 microsatellite loci OarAE101, OarHH35,BM143and BMS2508 which were closely linked to the fecundity gene \$FecB\$ in Booroola sheep was conducted in 159 sheep in Small Tail Han sheep, Dorset sheep, F 1 hybzids(Dorset×Small Tail Han sheep). The results confirmed the genetic characteristics of codominance of microsatellite DNA. The PCR amplified products of microsatellites were detected by non denatured (natural ) polyacrylamide gel electrophoresis. The sequences of PCR amplification fragment of 6 clones of 4 microsatellite loci in Small Tail Han sheep were accepted by GenBank of National Center for Biotechnology Information, National Institutes of Health in USA, the GenBank accession numbers were AF394445,AF394446, AF394447, AF394448, AF394449, AF394450, respectively. The sequence homogeneity between OarAE101 in Small Tail Han sheep in this study and the ovine OarAE101 in GenBank was 98 percent. The sequence homogeneity between OarHH35 in Small Tail Han sheep in this study and the ovine OarHH35 in GenBank was 99 percent. The sequence homogeneity between BM143 in Small Tail Han sheep in this study and the bovine BM143 in GenBank was 95 percent. The sequence homogeneity between BMS2508 in Small Tail Han sheep in this study and the bovine BMS2508 in GenBank was 95 percent. OarAE101,OarHH35,BM143 and BMS2508 in Small Tail Han sheep were all perfect microsatellites. These results could provide molecular basic data for the research on the germplasm characteristics of Small Tail Han sheep.

关 键 词：小尾寒羊 微卫星座位 克隆 序列分析 

分 类 号：S826[农业科学—畜牧学]