DNA和SDS介导钯卟啉室温磷光及磷光激发光谱分裂  

SDS and DNA-mediated Room Temperature Phosphorescence of Pd-porphyrin and Splitting of Excitation Band

作　　者：晋卫军[1] 李改茹[1] 

机构地区：[1]山西大学化学化工学院分析科学与药学系,山西太原030006

出　　处：《山西大学学报（自然科学版）》2002年第2期129-133,共5页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金 (2 9875 0 16 );山西省自然科学基金 (9910 10 );国家教育部课题

摘　　要：利用室温磷光研究了钯卟啉 (Pd- TAPP)与 ct DNA及 SDS的相互作用特征。 SDS通过静电缔合及尾链保护增强 Pd- TAPP磷光强度 ;Pd- TAPP通过静电和疏水相互作用结合于 DNA的沟槽区 ,缔合常数为 4.37× 10 5L· m ol- 1 (bp)。平均一个钯卟啉分子与 4～ 5个核酸碱基对相互作用时 ,核酸表面的钯卟啉分子达到饱和 ;然后卟啉分子开始聚集组装 ,激子相互作用导致激发光谱分裂 ,聚集缔合常数为 0 .5× 10 5L· mol- 1 。The interaction of meso-tetrakis (4-trimethylaminophenyl) porphyrin palladium (Pd-TAPP) with ct DNA and SDS was studied by the room temperature phosphorometry. The phosphorescence intensity (I RTP) of Pd-TAPP was increased by the electrostatic interaction and end-chain protection of SDS, and Pd-TAPP was bound to the grooves of nucleic acid by the electrostatic and hydrophobic interactions. The association constant was 4.37×10 5 L·mol -1(bp). The porphyrin molecules on the surface of nucleic acid reached saturation when one phosphor was bound to about 5 base pairs of nucleic acid. At that time, the porphyrin began to aggregate or assembly and the excitation spectra were splitted because of excitonic interaction. The aggregated constant was 0.5×10 5 L·mol -1.

关 键 词：DNA SDS 磷光激发光谱分裂 室温磷光 钯卟啉 核酸 作用机理 聚集行为 

分 类 号：O614.823[理学—无机化学] O657.3[理学—化学]