猫外侧膝状体神经元HRP示踪结合生长抑素的免疫组织化学研究  

THE STUDY OF HRP RETROGRADE TRACING COMBINED WITH SOMATOSTATIN IMMUNOHISTOCHEMISTRY ON NEURONS OF CAT LATERAL GENICULATE NUCLEUS

作　　者：卢大华[1] 曹启林[1] 罗学港[1] 

机构地区：[1]湖南医科大学神经生物学研究室,长沙410078

出　　处：《中国组织化学与细胞化学杂志》2000年第1期9-11,共3页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金资助项目!(No:392 70 2 6 9)

摘　　要：本文采用 HRP逆行示踪的方法 ,在猫视皮质的 1 7区多点微量注射 30 % HRP,逆行标记外侧膝状体核(L GN)至视皮质的中继神经元 ,继用免疫金银法作生长抑素 (SS)免疫组织化学 ,试图双标记 L GN的中继神经元。结果显示 :光镜下 HRP标记细胞与 SS免疫阳性细胞清晰可辨 ,HRP标记细胞内为较粗的棕色颗粒 ,分布于胞浆和树突基部 ;而 SS免疫阳性细胞内的颗粒为银染黑色颗粒 ;HRP和 SS双标记神经元内 ,上述两种颗粒共存。猫 L GN的 A、 A1 和 C板层均有SS免疫阳性神经元的分布 ;HRP标记细胞分布于 A和 A1 板层 ;双标记神经元位于 A和 A1板层 ;C板层未见。本文结合以前的研究认为 ,SS在 L GN至视皮质传导通路中的作用 ,可能与视觉信息的传递和调制有关。The HRP retrograde tracing technique combined with immunohistochemical method for somatostatin (SS) was used to detect the chemical properties of neurons in lateral geniculate nucleus (LGN). The results showed that the HRP labelled neurons were easily distinguished from SS immunoreactive cells. The particles of HRP labelled neurons were brown, while the immunogold particles of SS immunoreactive cells were black. The double stained neurons contained two kinds of particles mentioned. In cat LGN, the HRP labelled neurons were distributed in laminae A and A 1; The SS immunoreactive cells were located in laminae A,A 1 and C; The HRP and SS double labelled neurons were located in laminae A and A 1, and they constituted 37 5% of total number of HRP labelled cells. These results indicate that SS is probably involved in the transmission and modulation of visual information in the visual system.

关 键 词：猫 外侧膝状体 神经元 HRP示踪 生长抑素 免疫组织化学 视皮质 视觉系统 

分 类 号：Q436[生物学—生理学]