大鼠直肠肽能神经的支配与起源——HRP与免疫细胞化学法联合研究  

THE INNERVATION AND ORIGIN OF PEPTIDERGIC NERVESOF THE RECTUM IN THE RAT DOUBLE LABELING STUDY HRP RETROGRADE TRACING AND IMMUNOCYTOCHEMISTRY

作　　者：王秋桂[1] 殷光甫[2] 茹立强[2] 

机构地区：[1]咸宁医学院组织胚胎学教研室,咸宁437100 [2]同济医科大学神经生物学教研室,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2000年第3期337-343,共7页Chinese Journal of Histochemistry and Cytochemistry

基　　金：卫生部科学研究基金课题!( No. 96-1-13 9)

摘　　要：用 HRP追踪法与免疫细胞化学法观察了大鼠直肠内 P物质 (SP)、降钙素基因相关肽 (CGRP)和血管活性肠肽(VIP)三种肽能神经的支配与来源。结果显示 :1直肠 CGRP和 VIP肽能神经起源于盆丛副交感神经节 (PSG)。 2直肠感觉神经纤维来自骶 2～ 4节段双侧背根神经节 (S2 - 4 - DRG) SP能或 CGRP能神经元。 3感觉神经元的中枢突进入骶髓 2 -3节段后角并形成较粗大的外侧束 ,其中大部分传入纤维经后角外侧缘走行 ,终止于侧角区中间外侧核交感神经元胞体周围。其余部分传入纤维延伸到骶髓 2 - 3节段灰质第 、 层和灰质后连合核 (中央自主神经核 ) ,进入中间外侧核的传入纤维与后连合核也有联系。上述结果提示 ,支配直肠的 VIP能神经元参与了直肠肌运动的调节 ;SP和 CGRP能神经元可能与直肠的运动、感觉调节有关。The innervation and origin of the peptidergic nerves of three kinds( substance PSP;calcitonin gene- related peptide,CGRP and vasoactive intestinal polypeptide,VIP) of the rat rectum were observed by the HRP retrograde tracing and immunocytochemistry double labeling.The results were as follows;( 1 ) The CGRP and VIPpepidergic nervesof the rectum were originated from parasympathetic ganglia in the pelvis. ( 2 ) Sensory nervous fibers of the rectum were originated from neurons of the SP and CGRP in the bilateral S2 -4 dorsal root ganglia.( 3) After entering the3rd segment of sacral spinal cord the central processes of sensory neurons formed a large lateral tract,mostfibers of which extended along the lateral border of the sacral posterior horn and ended around the parasympathetic neurons of intermediolateral nuclei in the back horn region,while some afferent fibers extended into the laminae and and commissural nuclei.The afferent fibers of the intermediolateral nuclei were related to the posterior commissural nuclei.These results suggest VIP- like neurons which innervate the rectum participate in the regulation of the intestinal muscle motion,while SP- and CGRP- like nerves may be associated with the regulation of motion and sensation of the rectum.

关 键 词：HRP 肽能神经 直肠 逆行追踪术 免疫细胞化学 大鼠 

分 类 号：Q484[生物学—生理学] R322.45[医药卫生—人体解剖和组织胚胎学]