粒单系造血祖细胞CFU-GM琼脂半固体培养的原位固定及鉴定染色  

IN SITU FIXATION AND STAIN IDENTIFICATION OF HUMAN CFU-GM

作　　者：姜蓉[1] 王亚平[1] 戴勤[1] 

机构地区：[1]重庆医科大学基础医学院组织胚胎学教研室,重庆400016

出　　处：《中国组织化学与细胞化学杂志》2000年第3期344-346,共3页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：用粒单系造血祖细胞 (CFU- GM)体外琼脂半固体培养法 ,琼脂块原位固定 ,Wright染色与细胞化学和免疫细胞化学技术 ,对培养人的骨髓 CFU- GM进行集落性质鉴定。结果表明 :Wright染色能区别集落的性质及细胞的分化程度 :CFU-GM的细胞中 PAS、POX呈阳性反应 ;CD14、CD15分别在单核细胞和粒细胞的胞浆或胞膜上表达。该方法对鉴定细胞集落类型、集落中细胞的分化与成熟程度是简便可靠的手段。By using the techniques of cell culture,in situ fixation and specific stain of human CFU- GM,the characteristics of CFU- GM were investigated. The results indicated that the character of colony of hematopoietic progenitor cells and the level of cell differentiation cluld be identified by routine, cytochemical and immunocytochemical staining.Our experimental study has provided a simplified and reliable method for reservation and identification of cultured CFU- GM.

关 键 词：琼脂半固体培养 鉴定 粒单系造血祖细胞 体外培养 原位固定 集落染色 

分 类 号：R329－33[医药卫生—人体解剖和组织胚胎学] R331.14[医药卫生—基础医学]