LPS诱导乳鼠心肌细胞p38蛋白激酶的激活与转位  

THE ACTIVATION AND TRANSLOCATION OF p38 MITOGEN-ACTIVATED PROTEIN KINASE IN CARDIOMYOCYTE OF NUDE MOUSE AFTER STIMULATION OF LPS

作　　者：张琳[1] 姜勇[1] 张璐[1] 朴英杰 赵克森[1] 

机构地区：[1]第一军医大学全军休克微循环重点实验室,广州510515 [2]第一军医大学全军休克微循环中心实验室,广州510515

出　　处：《中国组织化学与细胞化学杂志》2000年第2期131-133,共3页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金!重点课题 (NO39830 40 0 );面上项目(NO3980 0 0 74; NO39870 332 );军队杰出人才基金!(NO98J0 0 3);广东省

摘　　要：探讨 p38蛋白激酶信号传导通路在细胞中的特异性作用机制。应用共聚焦激光扫描技术观察心肌细胞中 p38蛋白激酶的分布及 L PS对其分布的影响。结果提示未受刺激静止的及 EGF刺激的心肌细胞中 ,p38在胞浆和胞核中荧光强度呈弥散性分布 ;L PS刺激 30分钟后 ,细胞核区的荧光强度明显增强 ,而胞浆区域的荧光强度降低。心肌细胞受 L PS刺激激活后 ,其 p38蛋白激酶由胞浆转位到胞核。To study the translocation process of p38 MAPK on the action of lipopolysaccharide(LPS) for elucidation of mechanism of specific signal transduction of p38 MAPK. Laser scanning confocal microscope technique was used to check the distribution of p38 MAPK in myocardial cells and the influence of LPS on its translocation. Results\ From the study with the method of immunofluorescence labeling, it was found that p38 spread all over the cytosol and nuclei in the resting cells or cells stimulated with EGF. The fluorescent intensity in the nuclei of cardiomyocytes enhanced, while that in the cytosol area reduced after stimulation for 30 minutes. Conclusion p38 MAPK moved to the nuclei of myocardial cells after the stimulation of LPS.\;

关 键 词：心肌细胞 激活 P38蛋白激酶 LPS 转位 鼠 

分 类 号：Q26[生物学—细胞生物学] Q556