外源氯化胆碱可提高小麦线粒体膜的流动性  

EFFECT OF CHOLINE CHLORIDE ON MEMBRANE FLUIDITY OF MITOCONDRIAISOLATED FROM ETIOLATED SEEDLINGS OF WHEAT

作　　者：刘世名[1] 季玉龙[1] 陈靠山[1] 彭正华[1] 张举仁[1] 张长铠[1] 梁峥[2] 

机构地区：[1]山东大学生命科学学院,山东济南250100 [2]中国科学院植物研究所,北京100093

出　　处：《生物物理学报》2002年第1期19-22,共4页Acta Biophysica Sinica

基　　金：863计划资助项目 (863 -819 -07 -07)

摘　　要：分别用ANS、DPH及16 -NS三种不同的标记物标记小麦黄化苗的线粒体 ,研究氯化胆碱 (cholinechloride,CC)对线粒体膜的荧光光谱、平均微粘度 (η )及ESR图谱的影响。结果表明 ,0.21 -1.79mmol·L-1 的CC均能显著降低线粒体膜的荧光强度、η 值及ESR图谱的序参数 (S)和旋转相关时间 (τc) ,表明CC可增加线粒体膜的流动性。Effects of choline chloride (CC) on fluorescence intensity, micro-viscosity () and ESR spectra of mitochondria from wheat etiolated seedlings were studied with the fluorescence labels, ANS and DPH and a spin label, 16NS. Results indicated that the concentration of CC from 0.21 to 1.79 mmol·L-1 decreased fluorescence intensity, micro-viscosity (), sequence parameter (S) and rotating correlative time (τc) of mitochondria from etiolated wheat seedlings. CC could therefore increase the fluidity of mitochondrial membrane. All these results suggested that CC could affect the biophysical characteristics and functions of mitochondria, which shed some light on the mechanism of increased resistances against stresses in plants by CC.

关 键 词：氯化胆碱 小麦 线粒体膜 膜流动性 

分 类 号：Q946.885.9[生物学—植物学] S512.1[农业科学—作物学]