大鼠硫酸腺嘌呤预处理心肌酶活性及SDH的电镜细胞化学观察  

OBSERVATION ON CHANGES IN THE ACTIVITIES OF MYOCARDIAL ENZYMES AND SDH ELECTRON MICROSCOPIC CYTOCHEMISTRY OF ADENINE SULPHATE PRECONDITIONING IN RATS

作　　者：袁玉林[1] 雷岳山[1] 郑汉巧[2] 冯晓祥[1] 谭政 张友云[1] 

机构地区：[1]湖北医科大学解剖学教研室,武汉430071 [2]湖北医科大学病理生理教研室,武汉430071

出　　处：《中国组织化学与细胞化学杂志》2000年第4期394-396,共3页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：本实验用硫酸腺嘌呤预处理方法与经典缺血预处理方法对比分析大鼠心肌酶活性及 SDH的电镜细胞化学结构。雄性 SD大鼠 2 4只 ,分 4组 ,即 组 :正常对照组 ; 组 :缺血再灌注组 ; 组 :经典缺血预处理组 ; 组 :硫酸腺嘌呤预处理组。检测肌酸激酶 (CK)、细胞色素氧化酶 (CCO)及琥珀酸脱氢酶 (SDH)的活性。结果显示 :与 组比 , 组、 组 CK漏出减少 ,CCO、 SDH活性增强 ,细胞超微结构保存良好。结果表明 :硫酸腺嘌呤预处理方法具有类似经典缺血预处理效应。To compare classical ischemic preconditioning with the method of adenine sulphate preconditioning, the changes of activities of enzyems and SDH were obsereved by using electron microscopic cytochemistry 24 male SD rats were divided into 4 groups: the normal control group (group Ⅰ), ischemic reperfusion group (group Ⅱ), ischemic preconditioning group (group Ⅲ, Murry method) and adenine sulphate preconditioning group (group Ⅳ) The activities of creatine kinase (CK), cytochrome oxidase (CCO) and succinic dehydrogenase (SDH) were also detected The resluts shoned that the leaking out of CK in groups Ⅲ,Ⅳ was decreased, the activities of CCO, SDH in groups Ⅲ,Ⅳ were enhanced and the ultrastructure of cardiac muscle cell in groups Ⅲ,Ⅳ was maintained better compared with group Ⅱ It indicated that adenine sulphate preconditioning has similar protective effect as ischemic preconditioning has \;

关 键 词：心肌酶活性 SDH 细胞化学 硫酸腺嘌呤 缺血预处理 心脏 肌酸激酶 细胞色素氧化酶 琥珀酸脱氢酶 大鼠 超微结构 

分 类 号：R542.2[医药卫生—心血管疾病] R972[医药卫生—内科学]