胰岛素可磁化固相放射免疫分析  

Radioimmunoassay for Insulin in Serum with Magnetic Separation of the Solid-phase

作　　者：李建华[1] 刘慎沛[1] 崔武任[1] 叶维新[1] 张建华[2] 金之欣[2] 

机构地区：[1]同济医科大学实验医学研究中心核医学研究室 [2]同济医科大学附属同济医院内科内分泌室

出　　处：《同济医科大学学报》1991年第1期49-52,共4页Acta Universitatis Medicinae Tongji

摘　　要：用胰岛素(INS)免疫豚鼠制得INS抗血清,通过氯胺T法碘化标记INS制备^(125)I-INS,结合本室研制的可磁化固相二抗分离试剂(DAMP),建立了血清INS可磁化固相放射免疫分析法(RIA)。本法灵敏度达0.1μU,回收率>95％,批内变异(CV)<2.3％,批间CV<7.4％。并具有不需离心,操作简便,分离迅速的特点。经临床初步应用,获得满意的结果。A new radioimmunoassay for insulin in serum with magnetic separation of the solid-phase is described. The insulin antiserum was obtained from immunizing guinea pig with insulin. 125I-insulin was labeled by use of the io-dinating reagent chloramine-T. The double antibody magnetizable part ides (DAMP) were developed by our laboratory. The lowest detection limit was 0.1 μU. The recovery of the method was more than 95%. The coefficients of variation within assay were less than 2.3% while those between assays were less than 7.4%. This method avoids the need for centrifugation, provides a simple, rapid and universal separation procedure, and has been applied clinically with satisfactory results.

关 键 词：胰岛素 放射免疫测定 磁力学 

分 类 号：R446.62[医药卫生—诊断学]