利用转基因技术创造甜菜耐盐新种质  

The creation of new sugar beet germplasm tolerant to salt by genetic transformation

作　　者：杨爱芳[1] 赵仕兰[1] 朱丽萍[1] 翟淑梅[1] 张举仁[1] 黄粤 翟晓灵 

机构地区：[1]山东大学生命科学学院,山东济南250100 [2]青岛市农科所,山东青岛266100

出　　处：《山东农业科学》2002年第2期3-6,9,共5页Shandong Agricultural Sciences

基　　金：国家 8 63项目 (2 0 0 1AA62 70 2 0 )资助

摘　　要：采用农杆菌介导法将来源于E coli的胆碱脱氢酶 (CDH)基因 (betA)和来源于拟南芥的Na+ /H+antiport基因分别转入 5个糖甜菜品种的丛生芽细胞 ,获得了转基因植株。在耐盐性筛选中 ,转基因植株存活率高 ,部分植株生长较好 ,表现出比对照植株明显提高的耐盐性。在 2 %～ 3%NaCl胁迫下 ,少数转基因植株仍生长正常 ,显示出高耐盐性。本研究表明 ,转入在不同盐胁迫反应途径中起关键作用的酶基因均可提高甜菜耐盐性 。From the tips of clump buds transformed with Agrobacterium tumefaciens, the transgenic plants with choline dehydrogenase gene ( betA ) from E coli or Na +/H + antiport gene from Arabidopsis thaliana were produced from 5 varieties of sugar beet In the examination of salt tolerance, the transgenic plants showed higher surviving frequency than ck, some of them grew vigorously and indicated stronger tolerant to salt than ck Under the stress of 2%-3%NaCl, the small part of the transgenic plants showed normal growth and high salt-tolerance As a result, the genes which played key roles in different metabolism pathway under salt stress could increase the salt tolerance of transgenic plants, therefore the plants with higher salt tolerance could be produced by transforming several salt tolerant genes

关 键 词：betA Na^+/H^+antiport基因 转基因 甜菜 耐盐性 农杆菌地 种质 E.COLI 胆碱脱氢酶 丛生芽细胞 种质 

分 类 号：S566.303.53[农业科学—作物学]