流行性感冒纯化疫苗的研究  

Preparaton of Purified Influenza Vaccine

作　　者：王敏文[1] 王志刚[1] 范宇红[1] 张玉辉[1] 张松青[1] 陶 航[1] 李晓波[1] 赵 越[1] 

机构地区：[1]长春生物制品研究所,长春130062

出　　处：《中国生物制品学杂志》2002年第3期164-166,共3页Chinese Journal of Biologicals

摘　　要：［摘 要］目的 研制安全有效的流感疫苗。方法 将A1、A3和B三株流感病毒分别接种鸡胚，取尿囊液，灭活后，经超滤浓缩再经Sepharose-4FF层析纯化，除菌过滤后制成疫苗。结果 该疫苗的主要技术指标：血凝素含量、卵清蛋白含量、总蛋白含量及细菌内毒素均符合 WHO规程要求标准［1］，人体接种后无副作用，抗体阳转率三株均达99％以上。结论 以该纯化工艺制备的流感疫苗安全有效。[Abstract] Objective To prepare a safe and effective influenza vaccine.Methods Three strains of influenza virus(Al,A3 and B)were inoculated into chick embryo,then allantoic fluid was concentrated by ul-trafiltration and purified by Sepharose 4FF charomtography. Results The hemagglutinin, ovalburnin and total protein contents met The WHO Requirements for Biologies. The antibody positive conversion rate of the subjects immunized with the vaccine reached above 99% ,and no side effect was observed. Conclusion The influenza vaccine purified by the method is safe and effective.

关 键 词：流行性感冒 纯化疫苗 免疫原性 安全性 

分 类 号：R511.7[医药卫生—内科学]