蝗虫微孢子虫对东亚飞蝗卵黄原蛋白含量的影响  

Effect of Nosema locustae on the content of vitellogenin of Locusta migratoria manilensis

作　　者：陈建新[1] 沈杰[1] 宋敦伦[1] 张龙[1] 严毓骅[1] 

机构地区：[1]中国农业大学植物保护学院,北京100094

出　　处：《昆虫学报》2002年第2期170-174,共5页Acta Entomologica Sinica

基　　金：国家自然科学基金资助项目 ( 3970 0 0 98)

摘　　要：采用免疫学方法 ,对东亚飞蝗Locustamigratoriamanilensis感染蝗虫微孢子虫Nosemalocustae后体内卵黄蛋白含量的变化进行了研究。结果表明 ,感病蝗虫与对照健虫相比 ,卵黄发生有严重障碍 ,脂肪体和卵巢中卵黄原蛋白或卵黄蛋白含量极低 ,导致感病雌虫丧失产卵能力。脂肪体中卵黄原蛋白含量最高峰健虫为 18 7mg mL ,而病虫只有 4 7mg mL ;血淋巴中卵黄原蛋白含量最高峰健虫为 7 6mg mL ,而病虫只有 2 6mg mL ;卵巢中卵黄蛋白含量最高峰健虫为 73 4mg mL ,而病虫只有 4 9mg mL。Changes in the content of vitellogenin in Locusta migratoria manilensis infected with Nosema locustae were determined by immunology method. The results indicated that vitellogenin did not accomplish in the locust infected, and the contents of vitellogenin in fat body, haemolymph and ovary were very low compared with the control. The maximum content of vitellogenin in fat body in the treatment was 4 663 mg/mL, while the value was 18 655 mg/mL in the control. The maximum content of vitellogenin in haemolymph in the treatment was 2 627 mg/mL, while the value was 7 603 mg/mL in the control. The maximum content of vitellogenin in ovary in the treatment was 4 927 mg/mL while the value was 73 367 mg/mL in the control.

关 键 词：蝗虫微孢子虫 东亚飞蝗 卵黄原蛋白 含量 卵黄发生 卵黄蛋白 

分 类 号：S433.2[农业科学—农业昆虫与害虫防治] S476.14[农业科学—植物保护]