蓖麻蚕线粒体基因组细胞色素氧化酶亚基Ⅲ的序列及其分子进化分析  

Characterization of the cytochrome oxidase subunit Ⅲ gene of mitochondrial DNA from the eri silkworm,\% Samia cynthia ricini\%

作　　者：魏兆军[1] 赵巧玲[1] 张志芳[1] 肖庆利[1] 王章娥[1] 何家禄[1] 

机构地区：[1]农业部家蚕生物技术重点开放实验室

出　　处：《昆虫学报》2002年第2期193-197,共5页Acta Entomologica Sinica

基　　金：国家自然科学基金资助项目 ( 39970 5 71)

摘　　要：测定了蓖麻蚕Samiacynthiaricini线粒体基因组 (mtDNA)含完整的细胞色素氧化酶亚基Ⅲ (COX3)、tRNA Gly和部分的NADH亚基Ⅲ (ND3)基因的DNA片段序列。COX3基因编码框包含 789个核苷酸 ,编码 2 6 2个氨基酸的蛋白质。通过同源性比较 ,发现COX3基因的 3′端比 5′端要保守 ,其编码的蛋白在C端有两个保守序列存在。COX3的下游为 6 6bp的tRNA Gly基因。蓖麻蚕的COX3与家蚕COX3同源性最高 ,核苷酸和氨基酸序列同源性分别是 80 2 %和 85 6 %。根据COX3氨基酸序列进行了 12种无脊椎动物的分子进化树分析 ,认为在采用线粒体基因序列进行分子进化分析时 ,应该综合考虑物种的繁殖模式及生态特点。The cytochrome oxidase subunit Ⅲ gene (COX3), tRNA Gly gene and partial ND3 gene of the eri silkworm,\%Samia cynthia ricini, \%were sequenced. The open reading frame of COX3 gene contains 789 nucleotides, encoding a putative protein of 262 amino acids. Comparison of the amino acid homology with COX3 genes of other eleven arthropods was made.The results showed that the conservation of C′ termination was higher than N′ termination in COX3, and there were two conservative regions in C′ termination of COX3 The COX3 gene of mtDNA in the eri silkworm was most closely related to that of the silkworm Bombyx mori, with the homology of 80 2% nucleotide and 85 6% amino acid respectively. Based on the alignment of amino acid sequences deduced from the COX3 genes, a phylogenetic tree of the twelve invertebrates was constructed. It was proposed that when the molecular evolution is analysed based on mitochondrial genetic sequences, the reproduction pattern and ecological characteristics should be considered.

关 键 词：蓖麻蚕 线粒体基因组 细胞色素氧化酶亚基Ⅲ 序列 COX3基因 分子进化 

分 类 号：Q966[生物学—昆虫学] Q343.35