八字地老虎血球细胞系的建立  

Establishment of a cell line from the hemocytes of Xestia c-nigrum L. (Lepidoptera: Noctuidae)

作　　者：李长友[1] 郑桂玲[1] 王晓云[1] 宋捷[1] 李国勋[2] 

机构地区：[1]东北农业大学农学院植物保护系,哈尔滨150030 [2]河北农业大学植物保护系,保定071001

出　　处：《昆虫学报》2002年第2期279-282,共4页Acta Entomologica Sinica

基　　金：国际科学基金 (C 2 5 31 1);河北农业大学资助项目 ( 9816 )

摘　　要：由八字地老虎Xestiac nigrum血细胞建立了一株细胞系 ,命名为NEAU Xc 96 0 716H ,原代培养 90余天 ,现已传至 70余代。细胞多为圆形 ,部分梭形 ,细胞群体倍增时间约为 6 3h。具有典型的鳞翅目昆虫染色体特征 ,数量多 ,形态为短杆状和球形。酯酶同工酶谱为 5条主带 ,与同种昆虫 (八字地老虎 )的胚胎细胞系 (NEAU Xc 730E)酯酶图谱稍有不同 ,而与草地夜蛾细胞系 (IPLB SF 2 1)的酯酶图谱完全不同。该细胞系可以被八字地老虎核型多角体病毒XcNPV感染 ,但感染率较低。A new insect cell line, NEAU Xc 960716H, was established from Xestia c nigrum larval hemocytes through successive passage over 70 generations since July 1996. The cells were classified into two types: spherical and spindle. The population doubling time of the cell line was about 63 hours. The chromosomes were condensed short rods and round, typical in lepidopteran cell lines. The isozyme pedigree of esterase was different from the embryonic cell lines NEAU Xc 730E of Xestia c nigrum and IPLB\|SF 21. The cell line was susceptible to Xestia c nigrum nuclear polyhedrosis virus (XcNPV), although at a low level.

关 键 词：八字地老虎 血球细胞系 建系 血细胞 昆虫 组织培养 

分 类 号：S433.82[农业科学—农业昆虫与害虫防治] Q962[农业科学—植物保护]