Effects of CO_2 Enrichment on Microstructure and Ultrastructure of Two Species of Freshwater Green Algae  

CO_2浓度变化对两种淡水绿藻的显微结构和超微结构的影响(英)

作　　者：夏建荣[1] 高坤山[1] 

机构地区：[1]汕头大学海洋生物研究所,汕头515063

出　　处：《Acta Botanica Sinica》2002年第5期527-531,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金(39830 060 )。~~

摘　　要：order to investigate the morphological response of freshwater green algae to elevated CO2 concentration, Chlamydomonas reinhardtii Dang and Scenedesmus obliquus Kutz were cultured with enriched CO2, and their microstructure and ultrastructure were examined by microscopy and electron microscopy. The effect of CO2 enrichment to 186 mumol/L, was insignificant on the shape and size of C. reinhardtii, but significant in reducing the volume of S. obliquus. High-CO2 increased the amount of chloroplast. The pyrenoids occurred in low-CO2-grown cells but not in high-CO2-grown ones and more starch granules were observed in the former.为了探讨淡水绿藻在适应CO2 浓度变化过程中细胞形态和结构的变化 ,通过普通显微镜和电子显微镜观察了在不同CO2 浓度培养下的莱因衣藻 (ChlamydomonasreinhardtiiDang)和斜生栅藻 (ScenedesmusobliquusK櫣ｔｚ)细胞。结果表明 ,CO2 浓度变化对莱因衣藻细胞体积没有明显的影响 ,但斜生栅藻在低浓度CO2 培养下细胞体积明显增大 ,并可见细胞内含有大量颗粒。两种绿藻细胞的超微结构显示 ,在低浓度CO2 培养下 ,细胞内叶绿体数目明显减少 ,并可见明显的淀粉盘包围的蛋白核 ;细胞内还可见大量的淀粉粒。而在高浓度CO2 培养下 ,这两种绿藻细胞内均未见明显的蛋白核和大量淀粉粒出现。

关 键 词：Chlamydomonas reinhardtii CO2 MICROSTRUCTURE PYRENOID Scenedesmus obliquus starch granules ULTRASTRUCTURE 

分 类 号：Q942[生物学—植物学]