聚合酶链式反应(PCR)荧光检测研究  

Study for Fluorescent Measurement of Polymerase Chain Reaction(PCR)

作　　者：吴坚[1] 曹文祺 

机构地区：[1]中国计量科学研究院,北京100013 [2]国家标准物质中心,北京100013

出　　处：《计量学报》2002年第2期151-156,共6页Acta Metrologica Sinica

摘　　要：本文研究了荧光能量传递技术 (fluorescenceresonanceenergytransfer,FRET) ,建立了一套基于该理论在聚合酶链式反应 (PCR)中用于荧光检测和溯源性研究的实验装置 。A fluorescence resonance energy transfer (FRET) first was developed and a set of experiment instrument was designed based on it, which is used for fluorescent measurement and traceability in polymerase chain reaction (PCR) technology. The instrument works well in test.

关 键 词：聚合酶链式反应 荧光能量传递技术 荧光检测 溯源性 实验装置 DNA 人工合成 

分 类 号：Q523[生物学—生物化学]