利用水稻功能基因SSR标记鉴定水稻种质资源  

Evaluation of Rice Germplasm Using SSR Markers of Functional Gene in Rice

作　　者：赵勇[1] 杨凯[1] Akbar Ali Cheema 翁跃进[1] 

机构地区：[1]中国农业科学院作物品种资源研究所,北京100081 [2]巴基斯坦核农业生物研究所

出　　处：《中国农业科学》2002年第4期349-353,共5页Scientia Agricultura Sinica

基　　金：国际原子能机构资助项目 (IAEA)

摘　　要：利用 16对水稻功能基因的SSR引物研究了 2 3份世界 5个国家不同来源的水稻种质资源的遗传多样性 ,共检测出 78个等位基因变异 ,每对引物可检测 2～ 10个等位基因变异 ,平均为 5 .2个 ,品种间遗传相似系数在0 .13～ 0 .6 4之间。UPGMA聚类分析表明 ,在相似系数 0 .13处可以将材料分为 2大类 ,来自巴西、日本和中国的粳稻全部聚为第Ⅰ类 ,巴西陆稻和原产科特迪瓦的陆稻聚在第Ⅰ类的第三小群 ;第Ⅱ类全为籼稻 ,来源于巴基斯坦的籼稻和 1个来源于韩国的籼稻分布在第Ⅱ类 ,说明水稻功能基因在不同亚种、不同产地来源和不同生态类型的水稻之间存在差异 ,也进一步证实水稻功能基因的SSR标记是研究水稻种质资源分类、地理分布、生态类型和系谱分析的有效工具。Sixteen SSR (simple sequence repeats) primers of functional genes in rice were used to detect genetic diversity among 23 rice germplasms from 5 countries in the world. The average number of alleles per SSR locus was 5.2 with a range from 2 to 10. Genetic similarities among the 23 rice accessions ranged from 0.13 to 0.64. UPGMA cluster analysis showed that the 23 rice accessions could be classified into two distinct classes at similarities coefficient 0.13. The Japonica rice from Brazil, Japan and China was classed into class Ⅰ and upland rice accessions from Brazil were classed into the same class. The Indica rice from Pakistan and Korea was classed into class Ⅱ. Consequently, function of gene's SSR markers could be used as a useful tool for measuring genetic diversity, assigning rice to geographical distribution, and analysing its ecotype and pedigree relationship.

关 键 词：水稻 功能基因 SSR标记 鉴定 种质资源 聚类分析 

分 类 号：S511[农业科学—作物学]