棉花子叶离体培养与植株再生  

In Vitro Plant Regeneration from Cotyledon Explant of Cotton (Cossypiun hirsutum)

作　　者：张海[1] 易永华[1] 高晓华[2] 邢宏宜[1] 贾涛[1] 

机构地区：[1]西北农林科技大学农学院棉花所,陕西杨凌712100 [2]宝鸡市农业学校,陕西宝鸡721000

出　　处：《西北农业学报》2002年第1期84-85,共2页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省自然科学基金资助 (96SM13 )

摘　　要：以棉花无菌苗子叶为外植体 ,就激素对其不定芽的分化及植株再生的影响进行了研究。实验结果表明 ,BA对子叶不定芽的诱导效果最好 ;TDZ次之 ;ZT和 KT不能诱导子叶形成不定芽 ,IAA与 BA配合使用 ,叶片不定芽分化频率明显提高 ;NAA与 BA配合使用叶片不定芽分化频率有所下降 ;2 ,4-D对子叶不定芽分化有明显的抑制作用。在分化培养基中添加 1 .5 mg/ L GA3有利于芽的伸长。不定芽伸长后在不含激素的 MS或 MS+0 .5 mg/ L NAA培养基上诱导生根 。Effects of hormones on differentiation of adventitious buds and plant regeneration were studied in cotyledon culture of cotton in vitro, BA was considered as the best growth regulator for adventitious bud differentiation within 4 cytokinins. The combination of IAA and BA raised the differentiation frequency obviously. The higher differentiation frequency of adventitious buds was obtained in the medium MS+4.0 mg/L BA+0.5 mg/L IAA, while in the media with NAA and BA, the differentiation frequency of adventitious buds reduced. However, 2,4 D had the inhibition effect on the bud differentiation GA 3 can promote the elongation of adventitious buds. The elongated shoots rooted in media MS without hormore or MS+0.5 mg/L IAA; and developed into the complete plant.

关 键 词：棉花 子叶离体培养 激素 植株再生 

分 类 号：S562.035.3[农业科学—作物学]