利用PCR-RFLP技术测定种猪的氟烷基因型  

Porcine halothane genotype determination by PCR-RFLP

作　　者：何志平[1] 吕学斌[1] 陈晓晖[1] 

机构地区：[1]四川省畜牧科学研究院,成都610066

出　　处：《西北农林科技大学学报（自然科学版）》2002年第2期48-50,共3页Journal of Northwest A&F University(Natural Science Edition)

基　　金：四川省科委"九五"重点科技攻关项目 (10 2 0 10 1)

摘　　要：采用 PCR- RFL P方法对 6 1头长白猪、5 6头大约克猪和 30头杜洛克猪进行了氟烷基因型检测。结果表明 :3个品种猪群所含的氟烷应激基因分别为 5 .73% ,0 .89%和 3.33%。建议在四川省种猪选育过程中 ,应将氟烷基因型的检测作为种猪性能测定的内容之一 ,及时淘汰携带氟烷基因的个体 。Halothane genotypes of 61 Landrace,56 Large white and 30 Duroc were determined by PCR RFLP.The results show that there exist Hal genes in three breeds and their gene frequency are 5.73%, 0.89% and 3.33%,respectively.It is suggested that Hal determination should be included in performance testing in order to cull individuals with Hal genes and ensure breeder quality.

关 键 词：PCR-RFLP技术 测定种猪 氟烷基因型 

分 类 号：S828.2[农业科学—畜牧学]