牛卵泡卵母细胞冷冻保存的研究  

Research on cryopreservation of bovine follicular oocytes

作　　者：于永生[1,2] 常万存[1] 李鹏 严娟 刘艳[1] 马世援[1] 李青旺[4] 

机构地区：[1]山东省农科院高新技术中心 [2]西北农林科技大学畜牧兽医学院,陕西杨陵712100 [3]山东中大胚胎中心 [4]西北农林科技大学畜牧兽医学院

出　　处：《西北农林科技大学学报（自然科学版）》2002年第2期51-54,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：山东省自然科学基金资助项目 (Q99D0 6)

摘　　要：研究了用 3种不同方法 (A法 :程序冷冻 ;B法 :超快速冷冻 ;C法 :玻璃化冷冻 )冷冻保存的牛 GV期卵母细胞的发育潜力。结果表明 ,解冻后 ,C组卵母细胞形态正常率显著高于 A、B组 ;3种方法冷冻的卵母细胞成熟培养后 ,平均卵丘扩展率为 6 5 % ,其中 C组最高 (6 9% ) ;3组冷冻卵母细胞的体外成熟率、体外受精率和卵裂率分别为 14 .3% ,17.1% ,2 1.6 % ;11.1% ,12 .6 % ,15 .1%和 5 .6 % ,9.1% ,11.1%。结果还表明 :玻璃化冷冻的卵母细胞损伤较轻。The developmental competences of bovine GV Stage oocytes cryopreserved by three methods (Method A:Conventional freezing procedures;Method B:ultrarapid freezing;Method C:Vitrification) were studied.After thawing,the morphological normal rate of C gronp was significantly higher than those of group A and B.The average cumulus expansion rate of all the cultured oocytes was 65% and that of group C was the highest(69%).The in vitro maturation rates were 14.3%,17.1% and 21.6%,and the fertilization rates were 11.1%,12.5% and 15.0%,and the oocyte cleavage rates were 5.6%,9.1% and 11.1% in group A,B,C oocytes cryopreserved respectively.The results showed that the injuries of vitrified oocytes were less severe.

关 键 词：牛 卵泡卵母细胞 冷冻保存 体外受精 

分 类 号：S823.34[农业科学—畜牧学]