用DNA探针检测我国栽培的无核葡萄及辅助育种初探  

Detecting the Seedless Characteristics of the Grapes in China with DNA Probe and DNA Marker Assistant Selection

作　　者：王跃进[1] 杨英军[1] 周鹏[2] 张剑侠[1] 王西平[1] 

机构地区：[1]西北农林科技大学园艺学院,杨凌712100 [2]热带作物生物技术国家重点实验室,海口571101

出　　处：《园艺学报》2002年第2期105-108,共4页Acta Horticulturae Sinica

基　　金：国家自然科学基金资助项目 ( 39970 5 2 4) ;农业部 948项目 ( 9810 43) ;高校博士点基金资助项目

摘　　要：用葡萄无核基因的特异探针 18bp (5’CCAGTTCGCCCGTAAATG3’)检测了我国栽培的 2 1个无核葡萄品种和 9个有核对照品种的无核性 ,证实了该探针可以对葡萄无核基因进行准确的检测和鉴定 ;通过该探针对有核×无核 (红地球×红光无核 )杂交后代无核性状的鉴定 ,并对照大田结果 ,证明了 18bp检测葡萄无核基因探针具有预测葡萄无核性状的作用。The paper reported the detection proficiency of the seedlessness in 21 seedless grape cultivars and 9 seeded ones using a specific primer which was a probe available for detecting seedless genes in grapes' genome. The results showed that the specific probe could be accurately used in the cultivars cultured in China. The preselection of seedlessness was also reached in cross combination 'seeded cultivars (Red Globe)×seedless one (Flame seedless)'progenies. It furthermore demonstrated that the seedlessness in grapes was controlled by multigenes and the specific genetic marker was linked to one of the seedless genes in grapes.

关 键 词：DNA探针 检测 无核葡萄 辅助育种 遗传标记 

分 类 号：S663.1[农业科学—果树学]