梨自交不亲和强度不同品种花柱S糖蛋白含量的差异  

Differences of S-glycoprotein Content in the Styles among Pear Cultivars Differing in Self-incompatible Strength

作　　者：张绍铃[1] 杨记磙[2] 李秀根[2] 平塚伸 Joseph Ngwela Wolukau 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]中国农业科学院郑州果树研究所,郑州450009 [3]三重大学生物资源学部,日本津市5148507

出　　处：《园艺学报》2002年第2期165-167,共3页Acta Horticulturae Sinica

基　　金：国家自然科学基金资助项目 ( 30 170 6 5 1) ;江苏省自然科学基金资助项目 (BK2 0 0 10 6 2 ) ;高校博士学科点专项科研基金资助项目 ( 2 0 0 10 30 70 12 )

摘　　要：提取梨 (Pyrus)自交不亲和强度不同品种花柱可溶性蛋白质 ,应用等电聚焦电泳法在凝胶板上分离出S糖蛋白 ,用图像解析法进行定量。结果表明 ,花柱总S糖蛋白含量因品种而异 ,自交不亲和强、弱及自交亲和的品种每 1μg花柱可溶性蛋白中含总S糖蛋白分别为≥ 1.3ng、 0 .7～ 0 .9ng、≤ 0 .7ng。不同的S基因所表达的S糖蛋白量差异显著 ,即使是同一S基因所对应的S糖蛋白量也因品种而异。Stylar soluble proteins were extracted from the whole style of pear cultivars differing in self-incompatibility (SI) strength and were separated by isoelectricfocusing polyacrylamide gel electrphoresis (IEF-PAGE) and each S-glycoprotein content was quantified by an image analyzer. Total S-glycoprotein (sum of two allelic-linked S-glycoproteins) content differed among cultivars. The cultivars which were strong SI, weak SI and sel-compatibility contained ≥1.3 ng, 0.7-0.9ng, and ≤0.7ng of S-glycoprotein per μg of stylar soluble protein, respectively. S -allele linked glycoprotein content differed greatly in various S -genotypes. Furthermore, the S-glycoprotein content was even different in cultivars with the same S genotype.

关 键 词：品种 花柱 差异 梨 自交不亲和性 S基因 S糖蛋白 

分 类 号：S661.2[农业科学—果树学]