东山九孔鲍病毒病病毒的分离观察  

Isolation and observation of "virus disease" virus of abalone in Dongshan, Fujian

作　　者：方莹[1] 黄印尧[2] 颜江华[1] 颜青[1] 吴文忠[2] 倪子绵[3] 

机构地区：[1]厦门大学生命科学学院抗癌研究中心,福建厦门361005 [2]厦门出入境检验检疫局,福建厦门361012 [3]厦门大学化学化工学院电镜室,福建厦门361005

出　　处：《台湾海峡》2002年第2期199-202,T003,共5页Journal of Oceanography In Taiwan Strait

摘　　要：运用电镜负染和超薄切片技术 ,在福建东山患病的九孔鲍的肾组织细胞、肝细胞和肠上皮细胞中可见有大小为 1 0 0nm的具包囊的六角形病毒颗粒 ,病鲍组织匀浆后 ,经蔗糖分步超速离心 ,RNA酶及DNA酶消化去除组织内DNA、RNA后 ,获得纯化的病毒颗粒 .又经蛋白酶K消化后得到病毒的核酸 ,以DNaseI或RNaseA分别消化 ,经 1 % (m/m)的琼脂糖凝胶电泳证实该病毒为DNA病毒 .Using ultramicrotomy technique and electron microscopy technique combined with negative staining method, hexagal particles containing capsule in size around 100nm were observed in renal cell, liver cell and enteron cell of diseased abalone sampled from Dongshan, Fujian. Homogenized tissue of diseased abalone was super centrifugalized in ladder sucrose medium, followed by the digestion of DNA and RNA in tissue using DNase and RNase. Pure virus particles were then obtained. Nucleic acid of the virus was achieved by digesting the virus particles using proteinase K. Purified nucleic acid was digesting by DNaseI and RNaseA, respectively. This virus was ascertained to be DNA virus by 1%( m/m ) agarose electrophoesis.

关 键 词：九孔鲍 病毒 分离 电镜 超薄切片技术 肝细胞 

分 类 号：S944.45[农业科学—水产养殖]