Excel规划求解法研究Cd^(2+)与BSA的结合平衡  

Study on the Binding Equilibrium between Cd^(2+) and BSA with Excel Program Solving Method

作　　者：刘德龙[1] 杜红莉[1] 肖凤娟[1] 

机构地区：[1]河北师范大学化学系,河北石家庄050091

出　　处：《计算机与应用化学》2002年第3期280-282,共3页Computers and Applied Chemistry

基　　金：河北省教委自然科学基金;河北省博士科研基金

摘　　要：利用Excel规划求解方法研究了Cd^(2+)与牛血清白蛋白的结合平衡。实验中选用Cd^(2+)离子选择性电极为指示电极研究了在pH=6.0条件下,Cd^(2+)与牛血清白蛋白(BSA)浓度均为10μmol/L数量级时的结合作用。实验数据的Scatchard图分析表明:Cd^(2+)在BSA中有一个强结合部位,2个弱结合部位。然后进一步利用Excel规划求解方法对实验数据进行求解。计算得到的稳定常数值分别为K_1=2.41I×10~6,K_2=0.97×10~5,K_3=2.05×10~5。The binding of Cd2+ to bovine serum album (BSA) has been studied by Cd2+ ISEs combined with Excel program solving method. The Scatchard plot analysis shows that there are two kinds of binding sites: one strong binding site and about two weak binding sites in 10^(-5)mol/L Cd2 + -BSA system (pH6.0) . The successive stability constants for the Cd2+-BSA system by Excel program solving method are K1 = 2.41×10~6 , K2 = 0.97×10~5, and K3 = 2.05 ×10~5 .

关 键 词：EXCEL 规划求解法 离子选择性电极 结合平衡 牛血清白蛋白 环境生物无机化学 镉(Ⅱ) 毒性 

分 类 号：R995[医药卫生—毒理学] X131[医药卫生—药学]