甘蓝型油菜FAD2基因cDNA片段的克隆和序列分析  

Cloning and Sequencing of a cDNA Sequence Encoding FAD2 from Brassica napus

作　　者：熊兴华[1] 官春云[1] 李栒[1] 王学军[1] 周小云[1] 李家洋[2] 

机构地区：[1]作物基因工程湖南省重点实验室,湖南长沙410128 [2]中国科学院遗传研究所,北京100101

出　　处：《湖南农业大学学报（自然科学版）》2002年第2期97-99,共3页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省科技厅资助项目 (97JKY10 0 5 )

摘　　要：为了提高油菜的油酸含量 ,继而培育高油酸油菜品种 ,从甘蓝型油菜成熟叶片中分离总 RNA,经反转录合成 c DNA第一条链 ,以此为模板进行多聚酶链式反应 ,产物为一长 6 5 4 bp的 DNA片段 .经克隆和序列测定表明 ,其核苷酸序列与 Gen Bank中甘蓝型油菜 FAD2基因在比较区的同源性达 10 0 % .To increase the oleate contents in rape and breed a rape variety of high oleate contents,the first strand of cDNA from the total RNA extracted from leaf of rapeseed( Brassica napus cv XY15) was synthsized and was used as a template for PCR reaction with oligonucleotide as a primer synthesized according to the FAD2 gene from oilseed rape. The PCR products were cloned into pGEM T Easy and sequenced. The results show that the cloned fragment contains 654 nucleotides,and shares a homology of 100% in comparison part with the reported data.

关 键 词：甘蓝型油菜 FAD2基因 cDAN片段 基因克隆 序列分析 PCR 品质改良 油菜脱氢酶 

分 类 号：S565.4[农业科学—作物学]