SCF和IL-6双基因共转染促进小鼠造血和免疫功能的恢复  

Stimulating recovery of hemopoietic and immunological functions by cotransferring IL-6 and SCF genes in irradiated mice

作　　者：张浩[1] 毛秉智[1] 董波[1] 陈肖华[1] 张军权[1] 罗庆良[1] 秦凤华[1] 

机构地区：[1]北京放射医学研究所,100850

出　　处：《中华放射医学与防护杂志》2002年第2期77-79,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目 (3 9870 716)

摘　　要：目的　探讨逆转录病毒介导的IL 6和SCF双基因共转染骨髓基质细胞对辐射小鼠造血和免疫功能的影响。方法　利用动物实验观察逆转录病毒介导的含IL 6和SCF双基因共转染的骨髓基质细胞对辐射小鼠外周血、骨髓有核细胞数、骨髓细胞CFU GM培养、脾脏淋巴细胞LTT、CD4 CD8等指标的影响。结果　表明转IL 6和SCF基因的骨髓基质细胞可以促进辐射小鼠造血和免疫功能的恢复。结论　IL 6与SCF双基因在骨髓基质细胞中获得表达 ,为进一步研究转基因骨髓基质细胞对造血调控的作用奠定了基础。Objective\ To explore the hemopoietic and immunological functions of retroviral\|mediated IL\|6 and SCF genes cotransferred into bone marrow stromal cells in irradiated mice. Methods\ IL\|6 and SCF cDNA were recombined with retroviral vector pLXSN by gene recombination technolgy.Number of peripheral blood and bone marrow cells,CFU\|GM of bone marrow cells,LTT and CD4/CD8 ratio of spleen were also assayed in vivo. Results\ The bone marrow stromal cells transferred with IL 6 and SCF genes could stimulate the recovery of hemopoietic and immunological functions in irradiated mice in vivo. Conclusion\ IL 6 and SCF genes are simultaneously expressed in bone marrow stromal cells,which provide a basis for studies on hematopoietic regulation by gene\|transfected bone marrow stromal cells.\;

关 键 词：骨髓基质细胞 IL-6 干细胞刺激因子 基因转染 免疫功能 SCF 造血功能 

分 类 号：R392.1[医药卫生—免疫学]