Flt3配体联合其他细胞因子对受照射小鼠免疫系统的作用研究  

Flt3 ligand accelerates the recovery of the immune system in irradiated mice

作　　者：许志祥[1] 张学光[1] 朱剑昆[1] 徐颖[1] 李颖[1] 丁天[2] 赵仁亮[1] 於葛华[1] 强亦忠[1] 

机构地区：[1]苏州大学生命科学院医学生物技术研究所免疫研究室,江苏215007 [2]上海复旦大学生化系

出　　处：《中华放射医学与防护杂志》2002年第2期86-89,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：国防科工委国防预研基金资助项目 (Y5 5 73 162 );国际原子能机构 (IAEA)基金资助项目 (CRP 9 0 2 5 )

摘　　要：目的　研究Flt3配体 (FL)在受照射小鼠免疫系统恢复中的作用。方法　采用F 80 0血细胞自动计数仪行外周血细胞计数及白细胞分类计数 ,流式细胞仪分析外周血淋巴细胞表型。结果　照射前应用FL或照射后联合应用FL、IL 11、EPO和G CSF等细胞因子 ,能有效地促进受照小鼠外周血白细胞和淋巴细胞的恢复 ,对CD8+ 细胞的恢复作用尤为明显 ,同时使CD4 + CD8+ 细胞比值较早恢复正常 ,提高受照射小鼠的生存率。结论　FL不但能促进辐射损伤机体造血功能的恢复 ,对免疫系统的恢复亦具有重要的作用。FL单独或与其他细胞因子联合应用 。Objective\ To study the immunoprotective potential of Flt3 ligand (FL) alone or in combination with other cytokines in irradiated mice. Methods\ The number of peripheral blood cells was counted by using F\|800 apparatus and the phenotype of lymphocytes was analyzed by flow cytometry.The pathological manifestations of spleen,thymus and lymph nodes were also evaluated. Results\ FL used alone before irradiation or in combination with other cytokines after irradiation could stimulate the recovery of peripheral white blood cells,especially CD8\++ T lymphocytes,and accelerate the recovery of ratio of CD4\++/CD8\++ T lymphocytes.FL reduced immune tissue damage and stimulated recovery of spleen,thymus and lymph nodes.The administration of FL to mice prior to irradiation and,to a lesser extent,after irradiation,significantly enhanced survival rate of animals. Conclusion\ FL can stimulate not only the recovery of hematopoiesis,but also the improvement of immune function in irradiated mice.FL alone or in combination with other cytokines exerts a distinct radio\|protection effect.\;

关 键 词：FLT3配体 细胞因子 小鼠 免疫系统 作用研究 辐射损伤 

分 类 号：R14[医药卫生—公共卫生与预防医学]