合并全身放射损伤对皮肤伤口组织细胞的抑制效应及W_(11)-a_(12)的促愈作用  

Effects of whole body γ irradiation on skin wound cells and the repaired-promoting action of W_(11)-a_(12)

作　　者：舒崇湘[1] 程天民[1] 阎国和[1] 冉新泽[1] 

机构地区：[1]第三军医大学预防医学系全军复合伤研究所

出　　处：《中华放射医学与防护杂志》2002年第2期90-93,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：国家 973重点基础研究发展规划资助项目(G19990 5 42 0 5 );重庆市院士基金资助项目 (98 5 0 81)

摘　　要：目的　研究全身放射损伤对皮肤伤口组织细胞的影响及W1 1 a1 2 促愈作用。方法　小鼠 6Gy照射后 ,于背部造成两个皮肤切口伤 ,观察伤后伤口截面愈合速度、伤口炎细胞、成纤维细胞和毛细血管数量及表皮细胞中碱性成纤维细胞生长因子含量的变化。结果　与单纯创伤组相比 ,照射后伤口截面愈合速度降低 ,伤口不同细胞数量均有抑制效应 ,但效应程度不同 ,由重到轻依次为炎细胞、血管内皮细胞、成纤维细胞和表皮细胞 ;表皮细胞中碱性成纤维细胞生长因子含量显著减少。W1 1 a1 2 使伤口截面愈合速度加快、细胞数量增加 ,并使表皮细胞中碱性成纤维细胞生长因子含量增加。结论　伤后伤口局部细胞数量减少和表皮细胞中碱性成纤维细胞生长因子含量下降是合并放射损伤后创面难愈的重要原因之一 ,W1 1 a1 2 对伤口的细胞游走、增殖及表皮细胞分泌碱性成纤维细胞生长因子的功能具有促进作用。Objective\ To study the effects of 6 Gy whole body γ irradiation on components of wound cells and the repair\|promoting action of W 11 a 12 , an extract from Periplaneta americana . Methods\ After mice were received 6 Gy gamma ray irradiation,the area of healing range in wound cross section,the cellular infiltration of wound and the content of basic fibroblast growth factor (bFGF) in wound epithelial cells were observed and the healing\|promoting effect of W 11 a 12 on the radiation\|impaired wound was investigated. Results\ The area of healing range in cross section was decreased,various infiltrated cells were all inhibited by radiation,but the range of inhibition was more or less different,and the descending order of severity was as follows:macrophages,vascular endothelial cells,fibroblasts and epithelial cells.The content of bFGF in epithelial cells was decreased.W 11 a 12 had benefical heal\|promoting effect on radiation\|impaired wound:it increased cellular infitration and promoted synthesis and secretion of bFGF in epithelial cells. Conclusion\ The depletion of wound cells is mainly responsible for the healing deficits of radiation\|impaired skin wound and W 11 a 12 enhances cell migration and proliferation and promotes synthesis and secretion of bFGF in epithelial cells.\;

关 键 词：放射损伤 皮肤伤口 组织细胞 抑制效应 W11-A12 伤口愈合 碱性成纤维细胞生长因子 

分 类 号：R818[医药卫生—放射医学]