辣椒辣素对动物神经源性膀胱氮能神经变化的影响  

An experimental study of nitrergic innervation in urinary bladder after spinal cord injury

作　　者：郑少斌[1] 黄昭明[1] 张鹏[1] 

机构地区：[1]第一军医大学附属南方医院肾脏疾病研究所泌尿外科,广州510515

出　　处：《中华泌尿外科杂志》2002年第5期285-287,共3页Chinese Journal of Urology

基　　金：广东省科学技术研究计划基金 (A19983 3 7)

摘　　要：目的　通过观察脊髓损伤动物膀胱内灌注辣椒辣素后膀胱氮能神经的变化 ,探讨氮能神经在病理性膀胱中的作用。　方法　成年健康SD大鼠 4 4只 ,随机分为正常组、假损伤组及脊髓损伤模型组。模型组横断第 8～ 9胸髓后饲养 4～ 5周。取部分模型组大鼠行膀胱内辣椒辣素 (1mmol/L)灌注。所有动物膀胱基底部、体部及顶部分别进行ncNOS免疫组化检测。统计分析采用t检验。　结果　大鼠NOS阳性神经分布较少 ,模型组 (12 .8± 1.3)与正常组 (11.1± 0 .9)比较差别无显著性意义 (P >0 .0 5 ) ,辣椒辣素灌注后膀胱NOS阳性神经分布显著增加 (2 6 .1± 2 .7,P <0 .0 5 )。　结论　辣椒辣素灌注膀胱使膀胱氮能神经分布增加 ,提示辣椒辣素的作用机理与NO有关。Objective Rats bladder ncNOS IR following intravesical instillation of capsaicin after chronic spinal cord injury(SCI) was studied to investigate the mechanism of ncNOS IR in pathological bladder. Methods 44 adult Sprague Dawley rats were randomly divided into normal group,sham injury group and spinal cord injury group(4～5 weeks after T 8～9 spinalization).A dose of 1 mmol/L capsaicin was instilled in part of SCI rats.the distribution of ncNOS IR in bladder base,bladder body and bladder dome of all rats were examined. Results In rats,the ncNOS IR did not differ between the normal group (11.1±0.9) and the SCI group (12.8±1.3).Intravesical instillation of capsaicin significantly up regulated ncNOS IR in rat bladder of SCI group (26.1±2.7, P < 0.05 ). Conclusions In SCI rats,ncNOS IR can be significantly up regulated by intravesical capsaicin instillation,suggesting that NO may act as a factor in the action of capsaicin.Further studies are needed to determine exactly how NO acts on urinary bladder.

关 键 词：氮能神经变化 神经源性膀胱 大鼠 一氧化氮合酶 辣椒辣素 氮 

分 类 号：R694[医药卫生—泌尿科学]