问号澳洲型钩端螺旋体复合抗原基因ompL_1/flaB_2表达质粒的构建  

Construction of expression vector including the multi-gene encoding ompL_1 and flaB_2 from Leptospira interrogans serovar australis

作　　者：王敏[1] 戴保民[1] 游自立[1] 

机构地区：[1]华西医科大学病理生理学教研室钩端螺旋体病研究室,成都610041

出　　处：《中国人兽共患病杂志》2002年第3期10-12,共3页Chinese Journal of Zoonoses

基　　金：国家自然科学基金资助 (N0 3 9970 667)

摘　　要：目的　为增强澳洲型钩端螺旋体 (简称澳洲型钩体 ) 6 0 7株抗原的免疫原性 ,而构建其外膜蛋白抗原基因ompL1和内鞭毛抗原基因flaB2 的复合抗原基因重组质粒。方法　通过聚合酶链反应扩增ompL1及flaB2 ,将其分别克隆到pcD NA3 1/Myc-His(+)载体T7启动子下游 ,进行序列测定分析 ,在此基础上将ompL1及flaB2 同时克隆至表达载体 pcD NA3 1/Myc -His(+) ,构建成A - pcLMF1复合抗原基因表达质粒。结果　澳洲型钩体 6 0 7株的ompL1及flaB2 与报道的其它株钩体的序列呈很高的同源性。澳洲型钩体重组质粒A -pcLMF1经酶切鉴定证实 :有一 1 8kb片段插入。结论　澳洲型钩体复合抗原基因ompL1/flaB2Aim To construct a multi antigen gene recombinant plasmid including an outer membrane protein gene(ompL 1) and a flagellin B gene(flaB 2) to enhance the protective immunity of leptospira interrogans serovar australis strain 607 Methods The DNA fragments encoding ompL 1 and flaB 2 were amplified respectively by PCR,then they were cloned separately into the down stream of the T7 promoter of vector pcDNA3 1/Myc-His(+),and analyzed by DNA sequencing On the basis,the both gene were cloned into the same vector pcDNA 3 1/Myc-His(+),A-pcLMF1 recombinant plasmid of multi antigen gene was constructed Result Analysis of the DNA sequence of ompL 1 and flaB 2 from L australis strain 607 showed that the sequences were high level identity with other strains Conclusions A fusion expression plasmid containing multi antigenic gene ompL 1/flaB 2 from L australis was constructed

关 键 词：澳洲型钩端螺旋体 内鞭毛 外膜 复合抗原基因 钩端螺旋体病 融合表面质粒 

分 类 号：R377.5[医药卫生—病原生物学] R514.4[医药卫生—基础医学]