水蛭注射液对小鼠血栓形成及血浆纤溶酶原激活物及抑制物的影响  

Effect of leech injection on thrombosis and activity changes of plasma t-PA and PAI in mice

作　　者：沙建慧[1] 杨中万[1] 夏文春 

机构地区：[1]第四军医大学吉林军医学院生理教研室,吉林市132000 [2]吉林市医院血液内科,吉林市132013

出　　处：《中成药》2002年第3期198-199,共2页Chinese Traditional Patent Medicine

摘　　要：目的 :研究水蛭注射液对小鼠体内血栓形成和血浆纤溶酶原激活物 (t PA)及抑制物 (PAI)的影响。方法 :给不同组小鼠分别注射不同剂量水蛭注射液。用角叉菜胶诱发小鼠体内血栓形成。发色底物法测定血浆t PA及PAI活性。结果 :水蛭组与血栓对照组相比 ,尾部血栓形成长度百分数明显缩短 ,PAI明显降低 (P <0 .0 5 )。结论 :水蛭注射液具有抗栓作用 。Objective: To investigate the effect of leech injection on thrombosis and plasma tissue plasminogen activator (t PA) and its inhibitor (PAI) in the thrombosis model of mice. Methods: The different dose leech injection of different doses were given to mice in different groups for 7 days respectively. Thrombosis was induced in mice by carrageenan. The plasma t PA and PAI activity were detected by chromogennic substracte analysis.Results: The average thrombosis length of tail in the groups of leech injection were significantly shorter than the length in the groups of thrombosis control. Compared with the group of thrombosis control, the plasma PAI activity in the leech injection groups was lower. Conclusions: The leech injection can reduce thrombosis in mice. The mechanism might be associated with reducing the release of PAI.

关 键 词：水蛭注射液 血栓形成 角叉莱胶 纤溶酶原激活物 纤溶酶原激活物抑制物 中药 动物实验 

分 类 号：R285[医药卫生—中药学]