双氢青蒿素对卡氏肺孢子虫肺炎大鼠肺泡巨噬细胞凋亡的影响  

Effects of dihydroartemisinin on apoptosis of alveolar macrophages in rats infected with Pneumocystis carinii pneumonia

作　　者：李文桂[1] 陈雅棠[1] 刘成伟[1] 陈 蕊[1] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,重庆400016

出　　处：《免疫学杂志》2002年第3期207-210,共4页Immunological Journal

基　　金：国家自然科学基金资助项目(39670658)

摘　　要：目的 检测双氢青蒿素对卡氏肺孢子虫肺炎（PCP）大鼠肺泡巨噬细胞凋亡的影响。方法 以醋酸可的松皮下注射Wistar大鼠建立PCP动物模型,用60mg/kg双氢青蒿素治疗实验大鼠,杀鼠取肺,用胶原酶消化法分离肺泡巨噬细胞,用PI和TUNEL法检测其凋亡,同时设有正常大鼠对照组。结果 感染组和治疗组大鼠肺泡巨噬细胞凋亡率显著高于正常对照组,治疗组大鼠肺泡巨噬细胞凋亡率明显低于感染组。结论 卡氏肺孢子虫感染引起大鼠肺泡巨噬细胞发生凋亡,经双氢青蒿素治疗后PCP大鼠肺泡巨噬细胞凋亡降低。ve To detect the effects of dihydroartemisinin(DHA) on apoptosis of alveolar macrophages( AMs) in rats infected with Pneumocystis carinii pneumonia(PCP). Methods Wistar rat model of PCP was established by subcutaneous injection with cortisone ace-tate. Rats in experimental group were treated by 60 mg/kg DHA and killed to obtain lungs, AMs were separated by collagenase digestion and cultured by LPS stimulation, AMs' apoptosis was detected by PI and TUNEL methods respectively, normal rats served as control. Results Apoptotic rate of AMs in the infection or treatment group was obviously higher than that in normal control, but apoptotic rate of AMs in treatment group was remarkably lower than that in the infection group. Conclusion Apoptosis may be caused in AMs of PCP rats, it may be decreased after DHA therapy.

关 键 词：双氢青蒿素 卡氏肺孢子虫肺炎 肺泡巨噬细胞 细胞凋亡 动物实验 抗寄生虫病药 

分 类 号：R563.1[医药卫生—呼吸系统]